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Isocitrate dehydrogenase (IDH1) (NM_005896) Human Over-expression Lysate

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SKU
LY401782
Transient overexpression lysate of isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210582
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005896, NP_005887
Synonyms HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD
Predicted MW 46.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005887
Locus ID 3417
Cytogenetics 2q34
Protein Families WB
Protein Pathways Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways
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Other "Isocitrate dehydrogenase" proteins (19)
SKU Description Size Price
PH310582 IDH1 MS Standard C13 and N15-labeled recombinant protein (NP_005887) 10 ug
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LC401782 Lyophilized IDH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP310582 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), 20 µg 20 ug
$737.00
TP700038 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132H),with C-terminal Myc-DDK tag, expressed in HEK293 cells 20 ug
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TP700039 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132C),with C-terminal Myc-DDK tag, expressed in HEK293 cells 20 ug
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TP700040 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132L),with C-terminal Myc-DDK tag, expressed in HEK293 cells 20 ug
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TP700041 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132S),with C-terminal Myc-DDK tag, expressed in HEK293 cells 20 ug
$900.00
TP700042 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 mutant R132G),with C-terminal Myc-DDK tag, expressed in HEK293 cells 20 ug
$900.00
TP710042 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), with C-terminal polyhistidine tag, expressed in sf9 cells. 20 ug
$540.00
TP710043 Recombinant protein of mutant(R132H) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. 20 ug
$540.00
TP710044 Recombinant protein of mutant(R132C) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells 20 ug
$540.00
TP710045 Recombinant protein of mutant(R132L) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. 20 ug
$540.00
TP710046 Recombinant protein of mutant(R132S) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. 20 ug
$540.00
TP710047 Recombinant protein of mutant(R132G) of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1),with C-terminal DDK tag,expressed in sf9 cells. 20 ug
$540.00
TP710048 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132H) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells. 20 ug
$540.00
TP710049 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132C) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells 20 ug
$540.00
TP710050 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132L) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells 20 ug
$540.00
TP710051 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132S) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells 20 ug
$540.00
TP710052 Recombinant protein of human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) and mutant(R132G) heterodimer, with C-terminal polyhistidine and DDK tags, expressed in sf9 cells 20 ug
$540.00

file-alt Citations

Antibody Validation for Western blot: By the User, for the User
Pillai-Kastoori, Heaton, Shiflett et al
J. Biol. Chem. (2019) 295 (4), 926-939 DOI: 10.1074/jbc.RA119.010472
AI Summary: OriGene products—including human recombinant IDH1 protein (TP310582), IDH1 over-expression lysate (LY401782), parental HEK293T lysate (LY500001), IDH1 knockout cell lysate (LC810112), parental HeLa control lysate (LC810Hela), and 2x SDS buffer—were used as standardized controls and reagents to validate antibody specificity and reproducibility in Western blot experiments.
Snippet: Isocitrate dehydrogenase (IDH1) (NM_005896) human recombinant protein (OriGene# TP310582), IDH1(NM_005896) human over-expression lysate supplied with parental HEK293T lysate (OriGene# LY401782;HEK293T LY500001;Lot #0076CF), IDH1 Knockout cell lysate (supplied with Parental HeLa control lysate) (Origene #LC810112, LC810Hela;Lot #1601), were mixed with either 2X protein loading buffer (PLB) (LICOR# 928-40004) OR 2x SDS buffer (OriGene) and denatured by boiling at 97oC for 5 minutes. …
On the Utility of Short Echo Time (TE) Single Voxel 1H-MRS in Non-Invasive Detection of 2-Hydroxyglutarate (2HG); Challenges and Potential Improvement Illustrated with Animal Models Using MRUI and LCModel
Heo, Kim, Lee et al
PLoS ONE (2016) 11 (1), e0147794 DOI: 10.1371/journal.pone.0147794
Cited by 12
AI Summary: OriGene contributed human IDH1 (Cat# NM_005896) and IDH2 (Cat# NM_002168) gene products, which were used for PCR amplification and subsequent cloning into lentiviral vectors to create IDH1/2 mutant-overexpressing glioma models in this study.
Snippet: Specifically, the genes for human IDH1 (GenBank accession number NM_005896) and IDH2 (GenBank accession number NM_002168) were obtained from Origene (Rockville, MD, USA), PCR amplified, and cloned into lentiviral vector CD526A–1 (System Biosciences ... Cite SaveSaving
FigureFigureFigure
Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
Wen, Cho, Yun et al
J. Neurochem. (2014)
Cited by 17
AI Summary: OriGene contributed to this study by providing human IDH1 (NM_005896) and IDH2 (NM_002168) gene products, which were used to generate wild type and mutant constructs for transfection and subsequent metabolomic analysis.
Snippet: Lentiviral vector construction and preparation The genes for human IDH1 (GenBank accession number NM_005896) and IDH2 (GenBank accession number NM_002168) were obtained from Origene (Rockville, MD, USA), PCR-amplified and cloned into ... Cite SaveSaving
Showing 1-3 of 3 papers.
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