BCAT1 Mouse Monoclonal Antibody [Clone ID: OTI3F5]
$447.00
In Stock*
Specifications
| Product Data | |
| Clone Name | OTI3F5 |
|---|---|
| Application | IHC, Simple Western, WB |
| Recommended Dilution | WB 1:500~2000, IHC 1:150, Simple Western 1:20-1:50 |
| Reactivity | Dog, Human, Mouse |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell. |
| Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide |
| Concentration | Lot dependent |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Predicted Protein Size | 42.8 kDa |
| Gene Name | branched chain amino acid transaminase 1 |
| Database Link | |
| Background | This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. provided by RefSeq |
| Synonyms | BCATC; BCT1; ECA39; MECA39; PNAS121; PP18 |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
| Protein Families | Druggable Genome |
| Protein Pathways | leucine and isoleucine biosynthesis, leucine and isoleucine degradation, Metabolic pathways, Pantothenate and CoA biosynthesis, Valine |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI3F5" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| CF504360 | Carrier-free (BSA/glycerol-free) BCAT1 mouse monoclonal antibody, clone OTI3F5 (formerly 3F5) | 100 ug |
$620.00
|
|
| TA504360AM | BCAT1 mouse monoclonal antibody, clone OTI3F5 (formerly 3F5), Biotinylated | 100 ul |
$509.00
|
|
| TA504360BM | BCAT1 mouse monoclonal antibody, clone OTI3F5 (formerly 3F5), HRP conjugated | 100 ul |
$509.00
|
|
| TA504360S | BCAT1 mouse monoclonal antibody, clone OTI3F5 (formerly 3F5) | 30 ul |
$200.00
|
|
Citations
DDX6 undergoes phase separation to modulate metabolic plasticity and chemoresistance
Bi, Li, Ren et al
Nat Commun (2025) 17 (1), 260 DOI: 10.1038/s41467-025-66966-4
Bi, Li, Ren et al
Nat Commun (2025) 17 (1), 260 DOI: 10.1038/s41467-025-66966-4
Cited by 6
AI Summary: OriGene’s BCAT1 antibody (TA504360S) was used in this study to detect BCAT1 protein levels, enabling the authors to assess how DDX6 knockout affects BCAT1 expression and contributes to metabolic reprogramming and chemoresistance in acute myeloid leukemia.
Snippet: BCAT1 (TA504360S, Origene Technologies;1:2000), FLAG (F3165, Sigma-Aldrich;1:2000), Goat Anti-Mouse IgG H&L (HRP) (ab6789, Abcam;1:2000), and Goat Anti-Rabbit IgG H&L (HRP) (ab6721, Abcam;1:2000). β-actin (3700S, Cell Signaling Technology;1:2000),
EZH2 Inhibition Induces an Integrated Stress Response Driving Glutamine-Dependent Vulnerability in TNBC
Porras, Fukano, Gironne et al
bioRxiv (2025)
Porras, Fukano, Gironne et al
bioRxiv (2025)
AI Summary: In this study, the OriGene BCAT1 antibody (Cat#TA504360) was used for immunohistochemistry to assess BCAT1 protein expression, contributing to the analysis of metabolic adaptations in triple-negative breast cancer following EZH2 inhibition.
Snippet: LAT1 Cell Signaling Technology Cat#32683;RRID: AB_3265513 CD98 Cell Signaling Technology Cat#47213;RRID: AB_2799323 ASNS Cell Signaling Technology Cat#92479;RRID: AB_3082991 BCAT1 (for immunoblot) Cell Signaling Technology Cat#12822;RRID: AB_2798035 BCAT1 (for IHC) Origene Cat#TA504360;RRID: AB_11126287 PSAT1 NOVUS Biological Cat#NBP1-32920;RRID
Bcat1 is controlled by Tsc2/mTORC1 pathway at expression levels and its deficiency together with Bcat2 inactivation suppresses the growth of a Tsc2-/- tumor cell line
Nishikawa, Mezawa, Kobayashi
Genes Cells (2023)
Nishikawa, Mezawa, Kobayashi
Genes Cells (2023)
Cited by 1
AI Summary: In this study, the OriGene Bcat1 (OT13F5, TA504360) mouse monoclonal antibody was used to detect Bcat1 protein levels, contributing to the investigation of how Bcat1 expression is regulated by the Tsc2/mTORC1 pathway in tumor cells.
Snippet: The tuberous sclerosis complex (TSC) gene products (TSC1/TSC2) negatively regulate mTORC1. Although mTORC1 inhibitors are used for the treatment of TSC, Save;The following primary antibodies were used: β-actin (13E5, Cell Signalling Technology, #4970, 1:2000 dilution) rabbit monoclonal antibody (mAb), Bcat1 (OT13F5, OriGene, TA504360, 1:1000 to 2000) mouse mAb
METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolism
Han, Dong, Leung et al
Cell Stem Cell (2023) 30 (1), 52-68.e13 DOI: 10.1016/j.stem.2022.12.006
Han, Dong, Leung et al
Cell Stem Cell (2023) 30 (1), 52-68.e13 DOI: 10.1016/j.stem.2022.12.006
Cited by 162
AI Summary: In this study, OriGene’s Anti-BCAT1 antibody (Cat# TA504360) was used to detect BCAT1 protein levels, helping to elucidate the role of METTL16 in regulating BCAA metabolism during leukemogenesis.
Snippet: Anti-METTL16 antibody Sigma-Aldrich Cat# HPA020352;RRID:AB_1853828 Anti-BCAT1 antibody OriGene Cat# TA504360;RRID: AB_11126287
Metabolon formation regulates branched-chain amino acid oxidation and homeostasis
Patrick, Gu, Zhang et al
Nat Metab (2022) 4 (12), 1775-1791 DOI: 10.1038/s42255-022-00689-4
Patrick, Gu, Zhang et al
Nat Metab (2022) 4 (12), 1775-1791 DOI: 10.1038/s42255-022-00689-4
Cited by 52
AI Summary: OriGene’s BCAT1 antibody (catalogue no. TA504360) was used in Western blot experiments to detect BCAT1 protein levels, contributing to the analysis of branched-chain aminotransferase isozymes in the study.
Snippet: The branched-chain aminotransferase isozymes BCAT1 and BCAT2, segregated into distinct subcellular compartments and tissues, initiate the catabolism of branched- Save;Western blot was performed as described44 using antibodies against DBT (Thermo-Fisher, catalogue no. … PA5-28301a), BCAT1 (OriGene Technologies, catalogue no. TA504360), BCAT2 (ABconal, catalogue no. …
Proteomic analysis of lung cancer cells reveals a critical role of BCAT1 in cancer cell metastasis
Mao, Chen, Lu et al
Theranostics (2021) 11 (19), 9705-9720 DOI: 10.7150/thno.61731
Mao, Chen, Lu et al
Theranostics (2021) 11 (19), 9705-9720 DOI: 10.7150/thno.61731
Cited by 45
AI Summary: In this study, OriGene's BCAT1 shRNA lentiviral particles were used to knock down BCAT1 expression in lung cancer cells, enabling the researchers to demonstrate BCAT1's critical role in promoting metastasis.
Snippet: tumors relative to para-tumor “normal” tissues 4, 5. However, similar studies at the proteome level on metastatic lung cancers are still missing. Amino acid metabolism plays critical roles in tumorigenesis and metastasis. Among them, the transamination reaction from branched-chain amino acids (BCAA) to α-ketoglutarate (α-KG) is catalyzed by either of two types of BCATs, the cytosolic BCAT1
Showing 1-6 of 14 papers.
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