Cancer

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-cJUN clone UMAB67 mouse monoclonal antibody. HIER ACCEL buffer (B22C-125) (pH8.7) at 110C for 10 min, UM800011 (1:100). Detection was done with Klear Mouse (C/N D52-18) DAB Kit.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NTRK1 (Cat# RC213047, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTRK1(Cat# TA806413). Positive lysates LY419273 (100ug) and LC419273 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFB (RC202426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFB. Positive lysates LY418732 (100ug) and LC418732 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSNK1E (RC202436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSNK1. Positive lysates LY403455 (100ug) and LC403455 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAX (RC204369, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with Rabbit polycl. Positive lysates LY408504 (100ug) and LC408504 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ERBB2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K8 (RC211171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K8 (1:500). Positive lysates LY401593 (100ug) and LC401593 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein KRAS from HELA lysates (0.5 mg/mL) using KRAS Mouse Monoclonal Antibody #TA801672. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein PRKAR1A from HEK293T lysates (0.2 mg/mL) using PRKAR1A Mouse Monoclonal Antibody #TA500641. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Mouse colon tissue using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB15, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Simple Western™ analysis of endogenous protein CDK2 from HELA lysates (0.5 mg/mL) using CDK2 Mouse Monoclonal Antibody #TA502935. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VHL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded human melanoma using anti-BMP4 mouse monoclonal antibody; an unrelated antibody of the same isotype was used as negative control. (Clone UMAB38, dilution 1:50; heat-induced epitope retrieval by 10mM citri

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IL6 plasmid (RC202078, hIL6), mouse IL6 plasmid (MR227281, mIL6), rat IL6 plasmid (RR201203, rIL6) using anti-IL6 antibody TA500067 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Simple Western™ analysis of endogenous protein AKT1 from HepG2 lysates (0.2 mg/mL) using AKT1 Mouse Monoclonal Antibody #TA504230. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human PTCH1 plasmid (RC216999, hPTCH1), mouse PTCH1 plasmid (MR227010, mPTCH1) using anti-PTCH1 antibody TA500123(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Anti-MYD88 mouse monoclonal antibody (TA502117) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MYD88 (RC202253).

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CTNNB1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Figure A, Western blot analysis of overexpressed lysates (10ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human STAT5A plasmid (RC205753, hSTAT5A), mouse STAT5A plasmid (MR210689, mSTAT5A) using anti-STAT5A antibody TA502736 (1:10000@1mg/ml). Figure B, Western blot analysis of the same hSTAT5A and mSTAT5A samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Simple Western™ analysis of endogenous protein CDK2 from HELA lysates (0.2 mg/mL) using CDK2 Mouse Monoclonal Antibody #TA502915. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-EGFR mouse monoclonal antibody. (UM500070; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 (TA500078) mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GLI1 mouse monoclonal antibody. (UM800063; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BRAF mouse monoclonal antibody. (TA500431). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, UM800076) (1:200)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY P16 (Cat# RC220937, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-P16(Cat# TA500036). Positive lysates LY400022 (100ug) and LC400022 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human MEF2C plasmid (RC220584, hMEF2C), mouse MEF2C plasmid (MR226865, mMEF2C) using anti-MEF2C antibody TA502565 (1:5000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-JUN mouse monoclonal antibody. (UM800005; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and MET-Knockout hela cells (KO, Cat# LC810144) were separated by SDS-PAGE and immunoblotted with anti-MET monoclonal antibody UM800082. Then the blotted membrane was stripped and reprobed with anti-β-actin (TA811000) as a loading control (1:500).

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human IL1B plasmid (RC202079, hIL1B), mouse IL1B plasmid (MR226719, mIL1B), rat IL1B plasmid (RR211023, rIL1B) using anti-IL1B antibody TA506443 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human GLI1 plasmid (RC201110, hGLI1), mouse GLI1 plasmid (MR227023, mGLI1) using anti-GLI1 antibody TA803967 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SHC1 (Cat# RC204362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHC1 (Cat# TA501056). Positive lysates LY401060 (100ug) and LC401060 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB2 (RC212583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB2. Positive lysates LY417979 (100ug) and LC417979 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MMP7 mouse monoclonal antibody. (UM800061; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PKMYT1 (Cat# RC222657, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1(Cat# TA500874). Positive lysates LY401358 (100ug) and LC401358 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FOSL1 (Cat# RC202104, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1 (Cat#TA500624).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PDGFRB mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TGFB1 (Cat# RC200973, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGFB1 (Cat# TA506583)(1:2000).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TP53 mouse monoclonal antibody. (UM500053; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17. Positive lysates LY411669 (100ug) and LC411669 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEF2C (RC220584, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEF2C. Positive lysates LY419349 (100ug) and LC419349 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HES1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SENP2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human ARNT plasmid (RC216724, hARNT), mouse ARNT plasmid (MR210673, mARNT) using anti-ARNT antibody TA501147 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Immunohistochemical staining of paraffin-embedded human normal ovary and Adenocarcinoma of the ovary tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB14, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TYMP mouse monoclonal antibody. (UM800027; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FGFR2 (Cat# RC217098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFR2(Cat# TA502799). Positive lysates LY400050 (100ug) and LC400050 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NTF4 (Cat# RC204535, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF4(Cat# TA500034). Positive lysates LY416817 (100ug) and LC416817 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1. Positive lysates LY401580 (100ug) and LC401580 (20ug) can be purchased separately from OriGene.