NOTCH1 Mouse Monoclonal Antibody [Clone ID: OTI3E12]
$447.00
In Stock*
Specifications
| Product Data | |
| Clone Name | OTI3E12 |
|---|---|
| Application | IF, IHC, WB |
| Recommended Dilution | WB: 1:1000; IHC 1;50; RPPA 1:75; IF: 1:100 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1 |
| Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide |
| Concentration | 0.2 mg/ml |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Predicted Protein Size | 270.6 kDa |
| Gene Name | notch receptor 1 |
| Database Link | |
| Background | Notch1 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development |
| Synonyms | AOS5; AOVD1; hN1; TAN1 |
| Reference Data | |
| Protein Categories | Cancer: Gynecologic, Cancer: Haematopoietic and Lymphoid, CAR-T Targets, Growth Factors, Membrane Proteins |
| Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway |
| Protein Pathways | Dorso-ventral axis formation, Notch signaling pathway, Prion diseases |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI3E12" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| CF500078 | Carrier-free (BSA/glycerol-free) Notch1 mouse monoclonal antibody, clone OTI3E12 (formerly 3E12) | 100 ug |
$620.00
|
|
| TA500078AM | Notch1 mouse monoclonal antibody,clone 3E12, Biotinylated | 100 ul |
$509.00
|
|
| TA500078BM | Notch1 mouse monoclonal antibody,clone 3E12, HRP conjugated | 100 ul |
$509.00
|
|
| TA500078S | Anti-Notch1 mouse monoclonal antibody, clone OTI3E12 (formerly 3E12) | 30 ul |
$200.00
|
|
Citations
DCLK1 isoforms and aberrant Notch signaling in the regulation of human and murine colitis
Roy, Ahmed, Stubbs et al
Cell Death Discov (2021) 7 (1), 169 DOI: 10.1038/s41420-021-00526-9
Roy, Ahmed, Stubbs et al
Cell Death Discov (2021) 7 (1), 169 DOI: 10.1038/s41420-021-00526-9
Cited by 22
AI Summary: In this study, the OriGene mouse anti-Notch1 NICD antibody (clone OTI3E12) was used to detect Notch1 signaling in tissue samples, helping to elucidate the relationship between Notch1 and DCLK1 isoforms in the regulation of colitis.
Snippet: Antibodies used were rabbit anti-DCLK1 (1:200, ab31704) and Anti-CR (1:250, ab37056) from Abcam, Cambridge, UK;mouse anti-Notch1 NICD (1:200, clone OTI3E12) from Origene, Rockville, MD;rabbit anti-Hes1 (1:200, #11988) and mouse anti-Ki-67 (1:200, #9449
Notch1 serves as a prognostic factor and regulates metastasis via regulating EGFR expression in hypopharyngeal squamous cell carcinoma
Tian, Liu, Liu et al
Onco Targets Ther (2018) 11, 7395-7405 DOI: 10.2147/OTT.S175423
Tian, Liu, Liu et al
Onco Targets Ther (2018) 11, 7395-7405 DOI: 10.2147/OTT.S175423
AI Summary: OriGene's anti-Notch1 polyclonal antibody was used for immunohistochemical detection of Notch1 expression in tissue samples, enabling the study to assess Notch1's role as a prognostic factor and its involvement in metastasis in hypopharyngeal squamous cell carcinoma.
Snippet: Slides were washed three times with PBS and then incubated with anti-Notch1 polyclonal antibody (diluted 1:200;OriGene Technologies, Inc., Rockville, MD, USA) or mouse anti-EGFR monoclonal antibody (diluted 1:1,000;Santa Cruz Biotechnology, Inc., Dallas, TX;The quality of tumor/adjacent noncancerous tissue was monitored under the guidance of a clinical pathologist. … Slides were washed three times with PBS and then incubated with anti-Notch1 polyclonal antibody (diluted 1:200;Santa Cruz Biotechnology, Inc., Dallas, TX, USA) at 4°C overnight and washed twice with PBS prior to incubation with a secondary antibody at room temperature for 30 minutes. …
Notch1-Hes1 signalling axis in the tumourigenesis of biliary neuroendocrine tumours
Harada, Sato, Ikeda et al
J. Clin. Pathol. (2013) 66 (5), 386-91 DOI: 10.1136/jclinpath-2012-201273
Harada, Sato, Ikeda et al
J. Clin. Pathol. (2013) 66 (5), 386-91 DOI: 10.1136/jclinpath-2012-201273
Cited by 30
AI Summary: In this study, the Notch1 (mouse IgG, 5 μg/ml, OriGene) antibody from OriGene was used for immunohistochemical analysis to assess Notch1 expression in biliary neuroendocrine tumour samples.
Snippet: μg/ml, Dako), Jagged 1 (Goat IgG, 0.5 μg/ml, Santa Cruz, California, USA), Notch 1 (mouse IgG, 5 μg/ml, OriGene, Rockville, Maryland ... This cell line was cultured in culture flasks with a standard medium (RPMI 1640, Life Technologies, Tokyo, Japan) supplemented with 10% fetal
Showing 1-3 of 3 papers.
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