Pre-B cell

Circular map for RC222515

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TGM2 (Cat# RC217353, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGM2 (Cat# TA809245). Positive lysates LY401458 (100ug) and LC401458 (20ug) can be purchased separately from OriGene.

Circular map for RC211823

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HP (RC223612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HP. Positive lysates LY401576 (100ug) and LC401576 (20ug) can be purchased separately from OriGene.

Circular map for MR203848

Circular map for MG226319

Circular map for RC214278L1

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNF4A (RC214914, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNF4A.

Circular map for RC212501L1

Circular map for RG222681

Ccl26 Mouse Gene Knockout Kit (CRISPR)

Coomassie blue staining of purified PDHA2 protein (Cat# TP305416). The protein was produced from HEK293T cells transfected with PDHA2 cDNA clone (Cat# RC205416) using MegaTran 2.0 (Cat# TT210002).

Circular map for MR203414L3

Circular map for MR209072L4

Circular map for MR209509L4

Circular map for RC211159L3

Circular map for RR200465L4

Simple Western™ analysis of endogenous protein PRKAR1A from HEK293T lysates (0.2 mg/mL) using PRKAR1A Mouse Monoclonal Antibody #TA500641. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein AKT1 from HepG2 lysates (0.2 mg/mL) using AKT1 Mouse Monoclonal Antibody #TA504230. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CRYAB (Cat# RC202718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB (Cat# TA500583). Positive lysates LY419682 (100ug) and LC419682 (20ug) can be purchased separately from OriGene.

Human Collagen VII (COL7A1) activation kit by CRISPRa

Mouse Scn9a activation kit by CRISPRa

Coomassie blue staining of purified IFNA21 protein (Cat# TP310115). The protein was produced from HEK293T cells transfected with IFNA21 cDNA clone (Cat# RC210115) using MegaTran 2.0 (Cat# TT210002).

Western blot validation of overexpression lysate (Cat# LY403535) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210450 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Simple Western™ analysis of endogenous protein SERPINA1 from HepG2 lysates (0.2 mg/mL) using SERPINA1 Mouse Monoclonal Antibody #TA500374. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-IL1A monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-HPmouse monoclonal antibody. (Clone UMAB10, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human TAGLN plasmid (RC215789, hTAGLN v2), human TAGLN plasmid(RC202448, hTAGLN v1), mouse TAGLN plasmid (MR202069, mTAGLN) using anti-TAGLN antibody TA503092 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT1 (RC220257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1. Positive lysates LY401580 (100ug) and LC401580 (20ug) can be purchased separately from OriGene.

Commercial recombinant Hu SIRT1 Protein 500ng/mL was coated on plate and incubated at 4°C for overnight. After blocking with 1%BSA, 2x serially diluted primary antibody TA809834 (0.78 to 50ng/mL) and 10000x diluted HRP conjugated goat anti-mouse IgG were used for detection. Signal/Background ratio=63.34, EC50=93.36ng/ml

Circular map for RC213277

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

Simple Western™ analysis of endogenous protein ACTN1 from HELA lysates (0.2 mg/mL) using ACTN1 Mouse Monoclonal Antibody #TA500072. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STAT1 (Cat# RC213858, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1 (TA500542).

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DGKA mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ESR1 (Cat# RC213277, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ESR1(Cat# TA503759). Positive lysates LY400046 (100ug) and LC400046 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein SERPINA1 from HepG2 lysates (0.2 mg/mL) using SERPINA1 Mouse Monoclonal Antibody #TA500374. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TOP2A (Cat# RC221568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOP2A(Cat# TA802384). Positive lysates LY420704 (100ug) and LC420704 (20ug) can be purchased separately from OriGene.