BIRC5 (NM_001168) Human Tagged ORF Clone
SKU
RC205935
BIRC5 (Myc-DDK-tagged)-Human baculoviral IAP repeat containing 5 (BIRC5/Survivin), transcript variant 1
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | BIRC5 |
| Synonyms | API4; EPR-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001168 |
| ORF Size | 426 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001168.1 |
| RefSeq Size | 2655 bp |
| RefSeq ORF | 429 bp |
| Locus ID | 332 |
| UniProt ID | O15392 |
| Cytogenetics | 17q25.3 |
| Domains | BIR |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Colorectal cancer, Pathways in cancer |
| MW | 16.2 kDa |
| Summary | This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. provided by RefSeq, Jun 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001168" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC205935L1 | Lenti ORF clone of Human baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1, Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC205935L2 | Lenti ORF clone of Human baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1, mGFP tagged | 10 ug |
$525.00
|
|
| RC205935L3 | Lenti ORF clone of Human baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1, Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC205935L4 | Lenti ORF clone of Human baculoviral IAP repeat containing 5 (BIRC5), transcript variant 1, mGFP tagged | 10 ug |
$525.00
|
|
| RG205935 | BIRC5 (tGFP-tagged) - Human baculoviral IAP repeat containing 5 (BIRC5/Survivin), transcript variant 1 | 10 ug |
$425.00
|
|
| SC119405 | BIRC5 (untagged)-Human baculoviral IAP repeat containing 5 (BIRC5/Survivin), transcript variant 1 | 10 ug |
$150.00
|
|
Citations
Dominant-negative ATF5 rapidly depletes survivin in tumor cells
Sun, Angelastro, Merino et al
Cell Death Dis (2019) 10 (10), 709 DOI: 10.1038/s41419-019-1872-y
Sun, Angelastro, Merino et al
Cell Death Dis (2019) 10 (10), 709 DOI: 10.1038/s41419-019-1872-y
AI Summary: OriGene contributed to this study by providing the FLAG-tagged human survivin cDNA plasmid (pCMV6-entry, #RC205935) and the first-strand cDNA synthesis kit, which were used for survivin overexpression experiments and cDNA synthesis, respectively.
Snippet: Plasmids FLAG-tagged human survivin cDNA cloned into a pCMV6-entry vector was obtained from Origene, Rock- ville MD (#RC205935) 1µg of mRNA was used for the synthesis of cDNA using the first-strand cDNA synthesis kit (Origene);;Plasmids. FLAG-tagged human survivin cDNA cloned into a pCMV6-entry vector was obtained from Origene, Rockville MD (#RC205935) 1 µg of mRNA was used for the synthesis of cDNA using the first-strand cDNA synthesis kit (Origene)
YM155 sensitizes non-small cell lung cancer cells to EGFR-tyrosine kinase inhibitors through the mechanism of autophagy induction
Dai, Shu, Chen et al
Biochim Biophys Acta Mol Basis Dis (2018) 1864 (12), 3786-3798 DOI: 10.1016/j.bbadis.2018.10.015
Dai, Shu, Chen et al
Biochim Biophys Acta Mol Basis Dis (2018) 1864 (12), 3786-3798 DOI: 10.1016/j.bbadis.2018.10.015
AI Summary: OriGene’s survivin cDNA was used to generate a retroviral expression vector for functional studies in this paper investigating how YM155 sensitizes non-small cell lung cancer cells to EGFR-tyrosine kinase inhibitors through autophagy induction.
Snippet: A three tandem tags (S/SBP/2xFLAG-tagged) was cloned into a retroviral vector pBabe-puro to generate pSBH285. The cDNA of survivin (OriGene, USA) was then cloned into retrovirus expression vector to produce pSBH286 by use of the GAT EWAY cloning system
Survivin Monoclonal Antibodies Detect Survivin Cell Surface Expression and Inhibit Tumor Growth in vivo
Fenstermaker, Figel, Qiu et al
Clin. Cancer Res. (2018) 24 (11), 2642-2652 DOI: 10.1158/1078-0432.CCR-17-2778
Fenstermaker, Figel, Qiu et al
Clin. Cancer Res. (2018) 24 (11), 2642-2652 DOI: 10.1158/1078-0432.CCR-17-2778
AI Summary: OriGene’s human survivin cDNA clone was used to generate a tagged survivin construct (hSurvivin-Myc/FLAG) for investigating survivin cell surface expression in cancer cells in this study.
Snippet: Subclone cultures were scaled up for production of purified IgG. DNA constructs. To generate hSurvivin-Myc/FLAG, human survivin fused to Myc and FLAG tags at its C-terminus was first excised from a commercial cDNA clone (OriGene)
Polysaccharide from Lentinus edodes combined with oxaliplatin possesses the synergy and attenuation effect in hepatocellular carcinoma
Zhang, Li, Wang et al
Cancer Lett. (2016)
Zhang, Li, Wang et al
Cancer Lett. (2016)
Cited by 95
AI Summary: OriGene's survivin cDNA was used in this study to overexpress survivin in cells, enabling the researchers to investigate the role of survivin in the anti-tumor effects of Lentinan and oxaliplatin combination therapy against hepatocellular carcinoma.
Snippet: Overexpression of survivin. Cells were transfected with survivin cDNA (OriGene, USA) using a Lipofectamine 2000 kit (Invitrogen, USA) as instructed by the manufacturer. Cells similarly transfected with empty plasmids served as the negative control. ... Cite SaveSaving
AMPK/mTOR-Mediated Inhibition of Survivin Partly Contributes to Metformin-Induced Apoptosis in Human Gastric Cancer Cell
Han, Gong, Wang et al
Cancer Biol. Ther. (2014)
Han, Gong, Wang et al
Cancer Biol. Ther. (2014)
Cited by 107
AI Summary: In this study, OriGene’s survivin overexpression plasmid (RC208445) and survivin siRNA (SR321835) were used to manipulate survivin expression in gastric cancer cells, helping to demonstrate that AMPK/mTOR-mediated inhibition of survivin partly contributes to metformin-induced apoptosis.
Snippet: Cite SaveSaving
Vertical blockade of the IGFR- PI3K/Akt/mTOR pathway for the treatment of hepatocellular carcinoma: the role of survivin
Ou, Lee, Lin et al
Mol Cancer (2014) 13, 2 DOI: 10.1186/1476-4598-13-2
Ou, Lee, Lin et al
Mol Cancer (2014) 13, 2 DOI: 10.1186/1476-4598-13-2
Cited by 46
AI Summary: OriGene products contributed to this study by providing survivin over-expression plasmids (RC209307) and survivin shRNA (TF314616) used to manipulate survivin levels in hepatocellular carcinoma cells, enabling the authors to assess survivin's role in mediating the response to IGFR/PI3K/Akt/mTOR pathway inhibition.
Showing 1-6 of 7 papers.
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