Plasmablast

Siva1 Mouse Gene Knockout Kit (CRISPR)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD45 (PTPRC) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800180) (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PTPRC (Cat# RC216590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRC (Cat# TA506567). Positive lysates LY401003 (100ug) and LC401003 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD19 (RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19. Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Circular map for RC200410

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

Circular map for RC202922

Circular map for RC208851

Coomassie blue staining of purified CD19 protein (Cat# TP302922). The protein was produced from HEK293T cells transfected with CD19 cDNA clone (Cat# RC202922) using MegaTran 2.0 (Cat# TT210002).

Circular map for RC204252

Circular map for RC203179

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human SIVA1 plasmid (RC215680, hSIVA1), mouse SIVA1 plasmid (MR201528, mSIVA1) using anti-SIVA1 antibody TA506830 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PTPRC (Cat# RC216590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRC (Cat# TA506567). Positive lysates LY401003 (100ug) and LC401003 (20ug) can be purchased separately from OriGene.

Circular map for RC216590

Circular map for RC215680

Circular map for RC217847

RC208851L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC208851L4V particle to overexpress human TNFRSF17-mGFP fusion protein.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIVA1 (RC215680, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIVA1. Positive lysates LY416652 (100ug) and LC416652 (20ug) can be purchased separately from OriGene.

The binding affinity values of IgG and DuetMab with amino acid residue substitutions of the heavy chain and kappa light chain variable domains and their combinations of some of the substitutions to CD4 and CD70 (OriGene TP300410), as measured using an Octet384 instrument. Figure cited from MAbs, PMID: 25730144

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD38 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800153) (1:300)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD38 (Cat# RC203179, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38 (Cat# TA804538)(1:2000). Positive lysates LY419752 (100ug) and LC419752 (20ug) can be purchased separately from OriGene.

RC208851L3 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC208851L3V particle to overexpress human TNFRSF17-Myc-DDK fusion protein.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIVA1 (RC215680, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIVA1. Positive lysates LY416652 (100ug) and LC416652 (20ug) can be purchased separately from OriGene.

Circular map for RC206980

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPRC (RC216590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRC. Positive lysates LY401003 (100ug) and LC401003 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD19 (RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19. Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD19 (RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19. Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD38 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800153) (1:300)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD38 (Cat# RC203179, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38 (Cat# TA804538)(1:2000). Positive lysates LY419752 (100ug) and LC419752 (20ug) can be purchased separately from OriGene.

Flow cytometric Analysis of living RBC-lysed rabbit peripheral blood cells, using anti-rabbit CD38 antibody (TA810515), (Red), compared to a nonspecific negative control antibody (TA180144), (green) and PBS, (blue). (1:100) (1:50)

RC202922L2 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC202922L2V particle to overexpress human CD19-mGFP fusion protein.

RC202922L3 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC202922L3V particle to overexpress human CD19-Myc-DDK fusion protein.

RC202922L4 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC202922L4V particle to overexpress human CD19-mGFP fusion protein.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow cytometric analysis of living 293T cells transfected with CD70 overexpression plasmid (RC200410), Red)/empty vector (PS100001, Blue) using anti-CD70 antibody (TA813221). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.