IHCAB20

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DDX58 (Cat# RC217615, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX58(Cat# TA506141). Positive lysates LY415370 (100ug) and LC415370 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RNH1 plasmid (RC208360, hRNH1), mouse RNH1 plasmid (MR207287, mRNH1) using anti-RNH1 antibody TA501981 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as Figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

SF9 cells lysate (5 ug, left lane) and SF9 cells lysate expressing human recombinant protein fragment (5 ug, right lane) corresponding to amino acids 995-1280 of human ABCB1 (NP_000918) were separated by SDS-PAGE and immunoblotted with anti-ABCB1.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGFB (RC202426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGFB. Positive lysates LY418732 (100ug) and LC418732 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ATG3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYNN (RC207080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYNN. Positive lysates LY412964 (100ug) and LC412964 (20ug) can be purchased separately from OriGene.

Figure from citation: Positive correlation between LSD1, c-Myc, β-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, β-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100 View Citation. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Simple Western™ analysis of endogenous protein KRAS from HELA lysates (0.5 mg/mL) using KRAS Mouse Monoclonal Antibody #TA801672. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded testis using anti-MAGE-A4 C/N TA505362 clone OTI1F9 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, anti-MAGE-A4 diluted to 1:50. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image 40x magnification.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD1C (Cat# RC218490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD1C(Cat# TA505411). Positive lysates LY419762 (100ug) and LC419762 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human CD163 plasmid (RC208557, lane 2), mouse CD163 plasmid (MR216798, lane 3) , rat CD163 plasmid (RR206586, lane 4) using anti-CD163 antibody TA506380 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human DPP4 plasmid (RC209466, lane 2), mouse DPP4 plasmid (MR222698, lane 3) using anti-DPP4 antibody TA500733 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Simple Western™ analysis of endogenous protein TUBB4A from HELA lysates (0.2 mg/mL) using TUBB4A Mouse Monoclonal Antibody #TA503129. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein NNMT from HELA lysates (0.5 mg/mL) using NNMT Mouse Monoclonal Antibody #TA502624. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein SAMHD1 from THP-1 lysates (0.1 mg/mL) using SAMHD1 Mouse Monoclonal Antibody #TA502157. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein PRKAR1A from HEK293T lysates (0.2 mg/mL) using PRKAR1A Mouse Monoclonal Antibody #TA500641. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded normal tonsil using ACTB antibody TA811000 clone OTI1 mouse monoclonal antibody. Protocol used HIER TEE pH9.0 (cat# B21-100)and anti-ACTB at 1:1000 dilution. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image 40x magnification.

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human IFIT1 plasmid (RC203320, lane 2), mouse IFIT1 plasmid (MR207393, lane 3) using anti-IFIT1 antibody TA500948 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Simple Western™ analysis of endogenous protein LMAN1 from HEK293T lysates (0.2 mg/mL) using LMAN1 Mouse Monoclonal Antibody #TA502137. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human ALOX15 plasmid (RC206621, lane 2), mouse ALOX15 plasmid (MR226258, lane 3) using anti-ALOX15 antibody TA504250 (1:500).

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human SAMHD1 plasmid (RC206013, lane 2) using anti-SAMHD1 antibody TA502024 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human ROMO1 plasmid (RC210655, lane 2), mouse ROMO1 plasmid (MR215950, lane 3) using anti-ROMO1 antibody TA505580 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human TLE1 plasmid (RC204037, lane 2), mouse TLE1 plasmid (MR210559, lane 3) using anti-TLE1 antibody TA800294 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Simple Western™ analysis of endogenous protein PFKP from MCF-7 lysates (0.1 mg/mL) using PFKP Mouse Monoclonal Antibody #TA503982. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KRT8 (Cat# RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8 (Cat# TA500021)(1:500).

Simple Western™ analysis of endogenous protein BCAT1 from HepG2 lysates (0.5 mg/mL) using BCAT1 Mouse Monoclonal Antibody #TA504360. The virtual lane view (left) shows the target (as indicated) at 1:20 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:20 dilutios of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TMEM173 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VHL mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAD1 (Cat# RC207226, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAD1 (Cat# TA500329). Positive lysates LY400290 (100ug) and LC400290 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IL6 plasmid (RC202078, hIL6), mouse IL6 plasmid (MR227281, mIL6), rat IL6 plasmid (RR201203, rIL6) using anti-IL6 antibody TA500067 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

HEK293T cells transfected with either RC202365 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAI2 antibody (TA800167), and then analyzed by flow cytometry.

Simple Western™ analysis of endogenous protein TRPM4 from Jurkat lysates (0.2 mg/mL) using TRPM4 Mouse Monoclonal Antibody #TA500381. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human RGS5 plasmid (RC206857, lane 2), mouse RGS5 plasmid (MR201631, lane 3) using anti-RGS5 antibody TA503075 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Simple Western™ analysis of endogenous protein AKT1 from HepG2 lysates (0.2 mg/mL) using AKT1 Mouse Monoclonal Antibody #TA504230. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein TIA1 from HELA lysates (0.1 mg/mL) using TIA1 Mouse Monoclonal Antibody #TA800657. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BTN3A2 (Cat# RC201183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN3A2(Cat# TA500730). Positive lysates LY402082 (100ug) and LC402082 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein DNM1L from HELA lysates (0.2 mg/mL) using DNM1L Mouse Monoclonal Antibody #TA506153. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein FERMT2 from HepG2 lysates (0.2 mg/mL) using FERMT2 Mouse Monoclonal Antibody #TA500504. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FCGR2A (Cat# RC205786, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A(Cat# TA500645). Positive lysates LY402871 (100ug) and LC402871 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human ID3 plasmid (RC200583, hID3), mouse ID3 plasmid (MR227292, mID3) using anti-ID3 antibody TA500713 (1:500@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human RBPMS plasmid (RC200248, hRBPMS), mouse RBPMS plasmid (MR201952, mRBPMS) using anti-RBPMS antibody TA800206 (1:10000@1mg/ml). Figure B, Western blot analysis of the same human RBPMS and mouse RBPMS samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human PRRX1 plasmid (RC210393, lane 2), mouse PRRX1 v2 plasmid (MR202408, lane 3) , mouse PRRX1 v1 plasmid (MR226699, lane 4) using anti-PRRX1 antibody TA803116 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human PTCH1 plasmid (RC216999, hPTCH1), mouse PTCH1 plasmid (MR227010, mPTCH1) using anti-PTCH1 antibody TA500123(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Simple Western™ analysis of endogenous protein LIMK1 from HELA lysates (0.2 mg/mL) using LIMK1 Mouse Monoclonal Antibody #TA503012. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CRYAB (Cat# RC202718, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYAB (Cat# TA500583). Positive lysates LY419682 (100ug) and LC419682 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human NPR3 plasmid (RC219453, lane 2), mouse NPR3 v2 plasmid (MR208587, lane 3), mouse NPR3 v1 plasmid (MR227616, lane 4), rat NPR3 plasmid (RR203170, lane 5) using anti-NPR3 antibody TA501021 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PANK2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.