TRPM4 Mouse Monoclonal Antibody [Clone ID: OTI10H5]

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SKU
TA500381
TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5)
  $447.00
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Specifications
Specifications
Product Data
Clone Name OTI10H5
Application IHC, Simple Western, WB
Recommended Dilution WB 1:500~2000, IHC 1:50; Simple Western 1:20-1:50
Reactivity Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human TRPM4 (NP_060106) produced in HEK293T cell.
Buffer PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide
Concentration 1 mg/ml
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Blue Ice
Predicted Protein Size 134.3 kDa
Gene Name transient receptor potential cation channel subfamily M member 4
Database Link
Background Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload.
Synonyms hTRPM4; LTrpC4; PFHB1B; TRPM4B
Reference Data
Protein Categories Cardiovascular diseases, Cytokines, Intracellular Proteins, Membrane Proteins
Protein Families Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
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Other "OTI10H5" antibodies (4)
SKU Description Size Price
CF500381 Carrier-free (BSA/glycerol-free) TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5) 100 ug
$620.00
TA500381AM TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5), Biotinylated 100 ul
$509.00
TA500381BM TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5), HRP conjugated 100 ul
$509.00
TA500381S TRPM4 mouse monoclonal antibody, clone OTI10H5 (formerly 10H5) 30 ul
$200.00

file-alt Citations

FGD3 mediates lytic cell death, enhancing efficacy and immunogenicity of chemotherapy agents in breast cancer
Zhu, Dai, Ghosh et al
J Exp Clin Cancer Res (2025) 44 (1), 299 DOI: 10.1186/s13046-025-03559-5
Cited by 1
AI Summary: OriGene’s TRPM4 antibody (TA500381) was used in this study to investigate the role of TRPM4 in the mechanisms of lytic cell death mediated by FGD3 in breast cancer cells.
Snippet: gy, 2144, RRID: AB_2210548), HMGB1 (Abnova, H00003146-M08, RRID: AB_518856), Cdc42 (Cell Signaling Technology, 2462, RRID: AB_2078085), ARP2(Cell Signaling Technology, 3128, RRID: AB_2181763), TRPM4 (OriGene, TA500381, RRID: AB_2208624), ERα (Santa Cruz Biotechnology, sc-8002, RRID: AB_627558), vinculin (Cell Signaling Technology, 4650, RRID: AB_10559207). Antibodies were probed with horseradish
FigureFigureFigure
Generation of two isogenic human iPSC lines (ZIPi013-B-1, ZIPi013-B-2) carrying a CRISPR/Cas9-mediated deletion of TRPM4
Haferkamp, Telugu, Krieg et al
Stem Cell Res (2024) 81, 103609 DOI: 10.1016/j.scr.2024.103609
Snippet: Differentiation marker Mouse anti-BRACHYURY 1:100 Thermo Fisher Scientific, Cat# 14–9770-82, RRID:AB_2573016 Protein of interest Mouse anti-TRPM4 1:5000 OriGene, Cat# TA500381, RRID:AB_2208624 Protein of interest control Rabbit anti-β-actin 1:1000 Cell Signaling Technology, Cat# 4967, RRID:AB_330288
Two Transient Receptor Potential Channels at Focal Adhesions
Mitsou, Carlson, Multhaupt et al
J Histochem Cytochem (2023) 71 (9), 221554231194119 DOI: 10.1369/00221554231194119
Cited by 4
AI Summary: OriGene’s anti-TRPM4 antibody (mouse 10H5/TA500381) was used in this study to investigate the localization of TRPM4 in focal adhesions of fibroblasts, helping to confirm TRPM4 as a component of these cell-matrix junctions.
Snippet: … These are the calcium channel Save;Other antibodies against TRPM4 were from OriGene, Rockville, MD, (mouse 10H5/TA500381, WB: 1:700, ICC: 1:100) and Santa Cruz Biotechnology, Inc., Heidelberg, Germany, (goat, sc-27540;WB: 1:200, ICC: 1:100), Secondary Alexa Fluor 488-nm, 568-nm, or
WB ICC
Roles of TRPM4 in immune responses in keratinocytes and identification of a novel TRPM4-activating agent
(Otsuka) Saito, Fujita, Toriyama et al
Biochemical and Biophysical Research Communications (2023) 654, 1-9 DOI: 10.1016/j.bbrc.2023.02.062
Cited by 8
AI Summary: OriGene’s anti-TRPM4 antibody (cat. No. TA500381) was used to detect TRPM4 protein expression in keratinocytes, enabling investigation of TRPM4’s role in immune responses in this study.
Snippet: The skin is a protective interface between the internal organs and environment and functions not only as a physical barrier but also as an immune organ. However, the Save;Proteins were identified using anti-TRPM4 antibodies (cat. No. TA500381;1:1000;Origene Technologies, Inc. USA) and anti-β-actin mouse monoclonal antibodies (cat. No. 3700;Cell Signaling Technology, Danvers, MA, USA)
WB
Transcriptional Profiling of Tumorspheres Reveals TRPM4 as a Novel Stemness Regulator in Breast Cancer
Verigos, Kordias, Papadaki et al
Biomedicines (2021) 9 (10) DOI: 10.3390/biomedicines9101368
Cited by 15
AI Summary: OriGene contributed to this study by providing the anti-TRPM4 antibody (cat. No. TA500381), which was used in western blot analyses to investigate TRPM4 expression in breast cancer stem cells.
Snippet: tern blot analysis, total protein quantitation was performed using the BCA Protein Assay (ThermoScientific, Waltham, MA, USA). The antibodies used were anti-TRPM4 antibody (1:5000) (cat. No.TA500381, Origene, Rockville, MD, USA), anti-Actin (1:10,000) (a.a. 50–70, MAB1501, clone C4, Merck-Millipore Darmstadt, Germany), anti-rabbit IgG-HRP-linked (1:2000) (cat. No. 7074, Cell Signaling Technology
FigureFigureFigure
WB
Electrophysiological Effects of the Transient Receptor Potential Melastatin 4 Channel Inhibitor (4-Chloro-2-(2-chlorophenoxy)acetamido) Benzoic Acid (CBA) in Canine Left Ventricular Cardiomyocytes
Dienes, Hézső, Kiss et al
Int J Mol Sci (2021) 22 (17) DOI: 10.3390/ijms22179499
Cited by 13
AI Summary: In this study, OriGene’s anti-TRPM4 antibody (Cat# TA322487) was used for Western blot experiments to detect and analyze TRPM4 protein expression in canine heart tissues and isolated ventricular cells.
FigureFigureFigure
Showing 1-6 of 12 papers.
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