PD-L1 (CD274) (NM_014143) Human Tagged Lenti ORF Clone
$600.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PD-L1 |
| Synonyms | B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1; PDCD1LG1; PDL1 |
| Vector | pLenti-C-Myc-DDK |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014143 |
| ORF Size | 870 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014143.2 |
| RefSeq Size | 1553 bp |
| RefSeq ORF | 873 bp |
| Locus ID | 29126 |
| UniProt ID | Q9NZQ7 |
| Cytogenetics | 9p24.1 |
| Domains | ig, IG |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| MW | 33.28 kDa |
| Summary | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014143" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC213071 | PD-L1 / CD274 (Myc-DDK-tagged)-Human PD-L1 / CD274 molecule (PD-L1 / CD274) | 10 ug |
$300.00
|
|
| RC213071L2 | Lenti ORF clone of Human CD274 molecule (CD274), mGFP tagged | 10 ug |
$600.00
|
|
| RC213071L3 | Lenti ORF clone of Human CD274 molecule (CD274), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC213071L4 | Lenti ORF clone of Human CD274 molecule (CD274), mGFP tagged | 10 ug |
$600.00
|
|
| RG213071 | PD-L1 / CD274 (tGFP-tagged) - Human PD-L1 / CD274 molecule (PD-L1 / CD274) | 10 ug |
$489.00
$500.00
|
|
| SC115168 | PD-L1 / CD274 (untagged)-Human PD-L1 / CD274 molecule (PD-L1 / CD274) | 10 ug |
$450.00
|
|
Citations
Cell-targeted PD-1 agonists that mimic PD-L1 are potent T cell inhibitors
Curnock, Bossi, Kumaran et al
JCI Insight (2021)
Curnock, Bossi, Kumaran et al
JCI Insight (2021)
Cited by 80
AI Summary: OriGene products, specifically the lentiviral vectors containing PDCD1 (PD-1) (RC210364L1) and CD274 (PD-L1) (RC213071L1), were used to generate cells expressing PD-1 and PD-L1, enabling the study of PD-1/PD-L1 interactions and the evaluation of PD-1 agonist ImmTAAI molecules in this research.
Snippet: Lentivirus production and transduction Lentiviral vectors containing PDCD1 (PD-1) and CD274 (PD-L1) were obtained from OriGene (RC210364L1 and RC213071L1, respectively). NucLight red (IncuCyte NucLight Red Lentivirus Reagent) was obtained from
Showing 1-1 of 1 papers.
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