Lentiviral Packaging Kits

Snippet: Lentiviral Packaging Kit Origene TR30037 Seahorse XF Cell Mito Stress Test Kit Agilent 103015-100 Dynabeads Protein A Thermo Fisher Scientific 10002D NEBNext Ultra II DNA Library Prep kit NEB E7645S

Snippet: th 50μg/mL uridine. DMEM without penicillin-streptomycin was used when performing transfections and transductions. Cells were subsequently transduced to stably express Cas9 endonuclease (ABM # K002, Origene #TR30037). Knockout of MFN1: Cas9-expressing PS1 90% cells were transfected with a plasmid containing two gRNAs for MFN1 (gRNA1: GUU

Snippet: , #PS100142), together with the Lenti- vpak packaging system (OriGene, #TR30037). Transfection was performed using TurboFectin 8.0 (OriGene) in a 6- well plate format

Snippet: was produced by transfection of HEK293T cells using the Lentiviral Packaging Kit 454 (Origene, TR30037). Viral supernatants were harvested at 48 hours and 72 hours, filtered through 455 a 0.45 µm filter

Snippet: Lentivirus suspensions were generated by transfecting 70-80% confluent HEK293T cells with lentiviral vectors using the lentiviral packaging kit (Origene Cat# TR30037) or the Trans-Lentiviral shRNA packaging kit (Horizon Discovery Cat# TLP5912)