PD-L1 (CD274) (NM_014143) Human Tagged ORF Clone
$489.00
$500.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PD-L1 |
| Synonyms | B7-H; B7H1; hPD-L1; PD-L1; PDCD1L1; PDCD1LG1; PDL1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014143 |
| ORF Size | 870 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014143.4 |
| RefSeq Size | 1553 bp |
| RefSeq ORF | 873 bp |
| Locus ID | 29126 |
| UniProt ID | Q9NZQ7 |
| Cytogenetics | 9p24.1 |
| Domains | ig, IG |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| Summary | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014143" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC213071 | PD-L1 / CD274 (Myc-DDK-tagged)-Human PD-L1 / CD274 molecule (PD-L1 / CD274) | 10 ug |
$300.00
|
|
| RC213071L1 | Lenti ORF clone of Human CD274 molecule (CD274), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC213071L2 | Lenti ORF clone of Human CD274 molecule (CD274), mGFP tagged | 10 ug |
$600.00
|
|
| RC213071L3 | Lenti ORF clone of Human CD274 molecule (CD274), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC213071L4 | Lenti ORF clone of Human CD274 molecule (CD274), mGFP tagged | 10 ug |
$600.00
|
|
| SC115168 | PD-L1 / CD274 (untagged)-Human PD-L1 / CD274 molecule (PD-L1 / CD274) | 10 ug |
$450.00
|
|
Citations
Unveiling the Intracellular Biology of PD-L1 in Non-Transformed and Cancer Lung Epithelial Cells
Letian
Thesis (2022)
Letian
Thesis (2022)
Snippet: Name Details Backbone PD-L1-GFP cloned from Origene vector RG213071 plenti6 PD-L1-APEX2 cloned from Origene vector RG213071 and Addgene vector 66170 plenti6 PD-L1 WT Cloned from PD-L1-GFP. Stop codon after terminal T290. plenti6 PD-L1 CD Cloned from PD-L1-GFP. Stop codon after K263 in cytoplasmic domain plenti6 CMTM6_shRNA targeting sequence: 5′- TTCAAAGAAGTAGAGGCCTCCA-3' LT3REPIR
Platelet PD-L1 reflects collective intratumoral PD-L1 expression and predicts immunotherapy response in non-small cell lung cancer
Hinterleitner, Strähle, Malenke et al
Nat Commun (2021) 12 (1), 7005 DOI: 10.1038/s41467-021-27303-7
Hinterleitner, Strähle, Malenke et al
Nat Commun (2021) 12 (1), 7005 DOI: 10.1038/s41467-021-27303-7
Cited by 177
AI Summary: OriGene products, specifically the human FN1 siRNA Oligo Duplex (SR320193) and the universal scrambled negative control siRNA (SR30004), were used to manipulate fibronectin expression and serve as experimental controls in studying the mechanisms of PD-L1 transfer from tumor cells to platelets in non-small cell lung cancer.
Snippet: of Fibronectin, Lipofectamine TM 3000 and 100 pmol of human (FN1) siRNA Oligo Duplex (Locus ID 2335) (Origene, SR320193) was used. As scrambled negative control we used 100pmol universal scrambled negative control siRNA (Origene, SR30004).
Phage display-identified PD-L1-binding peptides reinvigorate T-cell activity and inhibit tumor progression
Gurung, Khan, Gunassekaran et al
Biomaterials (2020) 247, 119984 DOI: 10.1016/j.biomaterials.2020.119984
Gurung, Khan, Gunassekaran et al
Biomaterials (2020) 247, 119984 DOI: 10.1016/j.biomaterials.2020.119984
Cited by 85
The splicing FK506-binding protein-51 isoform plays a role in glioblastoma resistance through programmed cell death ligand-1 expression regulation
D'Arrigo, Digregorio, Romano et al
Cell Death Discov (2019) 5, 137 DOI: 10.1038/s41420-019-0216-0
D'Arrigo, Digregorio, Romano et al
Cell Death Discov (2019) 5, 137 DOI: 10.1038/s41420-019-0216-0
Cited by 31
AI Summary: In this study, OriGene products—including FKBP5 siRNA (SR300363) and PD-L1 cDNA (RC217445)—were used to modulate gene expression, enabling the authors to investigate the roles of FKBP51s and PD-L1 in glioblastoma cell survival, stemness, and resistance.
Showing 1-4 of 4 papers.
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