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pCMV6-AC-GFP Mammalian Expression Vector

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SKU
PS100010
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
  $780.00
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Specifications
Specifications
Product Data
C-Tag TurboGFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal tGFP tag.
  • TurboGFP (excitation/ emission max = 482/ 502 nm) is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboGFP
  • Such clones are the best for monitoring protein expression, localization, translocation and co-localization with other protein(s).
  • If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal GFP-tagging vector, PS100019.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • AC_GFP_R Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Homozygous variants in ZSWIM6 cause severe syndromic short stature and developmental delay
Amaratunga, Bezdicka, Tayeb et al
Horm Res Paediatr (2026)
AI Summary: OriGene products, specifically the human wild-type ZSWIM6-tGFP plasmid (cat. no. RG227098) and the empty tGFP plasmid pCMV6-AC-GFP (cat. no. PS100010), were used to generate expression constructs and controls for in vitro functional studies of ZSWIM6 variants in this research.
Snippet: A commercially available plasmid harbouring human wild type ZSWIM6 gene tagged with turbo green fluorescent protein (cat. no. RG227098;OriGene, Rockville, USA, NCBI reference: NM_020928.2, NP_065979.1), an empty-tGFP plasmid pCMV6-AC-GFP (cat. no. PS100010, OriGene, Rockville, USA),
Mitochondrial dysfunction underlies cell cycle arrest and tubular hypoplasia in ClC-Kb-deficient Bartter syndrome mice
Hsieh, Chen, Sung et al
JCI Insight (2026)
AI Summary: OriGene products pCMV6-AC-GFP empty vector (PS100010), pCMV6-ACClC-Kb-GFP (RG228424), and pCMV6-AC-Barttin-GFP (RC213125) were used for plasmid transfection to study the effects of ClC-Kb and Barttin expression on cell function in this investigation of Bartter syndrome mechanisms.
Snippet: The cells were then transfected with 1 µg total plasmid DNA (1 µg pCMV6-AC-GFP empty vector [PS100010] or 0.5 µg pCMV6-ACClC-Kb-GFP [RG228424] plus 0.5 µg pCMV6-AC-Barttin-GFP [RC213125];Origene) using Lipofectamine 3000 (Thermo Fisher) in 500 µL of serum-free DMEM
Nuclear localization of the glutaminyl-tRNA synthetase QARS reveals its role in DNA damage response in ovarian cancer
Serafini, Fazio, Kieffer et al
Nature Communications (2026)
AI Summary: In this study, OriGene products—including the pCMV6-AC-GFP Mammalian Expression Vector (#PS100010), GFP-tagged QARS-wt (#RG201784), and GFP-tagged QARSK496A (#CW309404) plasmids—were used for QARS overexpression experiments to investigate its role in DNA damage response in ovarian cancer cells.
Snippet: For QARS overexpression experiment, 1.5 x105 RPE cells were plated in six -well plates and incubated overnight prior to transfection with 2.5 g of plasmid either encoding : GFP only (pCMV6 - AC -GFP Mammalian Expression Vector, Origene, #PS100010), or GFP -tagged QARS -wt (QARS1 NM_005051 Human Tagged ORF Clone, Origene #RG201784) or GFP -tagged QARSK496A 45 (QARS1 NM_005051 Human Tagged ORF Clone, Origene #CW309404), QARS constructs containing 3×NES
Activity-regulated circSamm50 modulates mitochondrial dynamics and spine structural plasticity
Chanda, Bapat, Wingfield et al
Cell Rep (2026) 45 (6), 117378 DOI: 10.1016/j.celrep.2026.117378
AI Summary: In this study, OriGene products pCMV6-AC-GFP (PS100010) and pCMV6-AC-RFP (PS100034) were used as fluorescent expression vectors to facilitate visualization and analysis of gene expression and protein localization related to circSamm50’s role in mitochondrial dynamics and synaptic plasticity.
Snippet: pCMV6-AC-GFP Origene PS100010 pGL3 Basic Luciferase vector Promega E1751 pXR001-mCD4: EF1a-RfxCas13d-P2A-mCD4-T2A-EGFP Addgene 228359 Samm50-WT-pGL3 This paper N/A Samm50-MUT-pGL3 This paper N/A circSamm50-WT- pGL3 This paper N/A circSamm50-MUT-pGL3 This paper N/A RCaMP1.07 This paper N/A pCMV6-AC-RFP Origene PS100034
Carboplatin alleviates astrocytic TDP-43 neurotoxicity by inhibiting NF-κB activation
Song, Lee, Lee et al
Eur J Pharmacol (2026) 1026, 178968 DOI: 10.1016/j.ejphar.2026.178968
AI Summary: OriGene products, specifically the pCMV6-AC-GFP (Cat# PS100010) and pCMV6-AC-TDP-43-GFP (Cat# RG210639) plasmids, were used to transfect astrocytes with GFP or wild-type human TDP-43, enabling the study of TDP-43-induced neurotoxicity and the protective effects of carboplatin in cellular models.
Snippet: Scientific). A total of 100 × 104 cells were electroporated with 5 μg of either green fluorescent protein (GFP) (pCMV6-AC-GFP;Cat# PS100010;OriGene Technologies), wild-type human TDP-43 (pCMV6-AC-TDP-43-GFP;Cat# RG210639;OriGene Technologies), or TDP-43 Q331K mutant plasmids
L-Leucine Upregulates Lysosomal Biogenesis and Autophagy to Lower Plaques in 5XFAD Mouse Model of Alzheimer's Disease
Paidi, Gorai, Mondal et al
J Neurochem (2026) 170 (4), e70432 DOI: 10.1111/jnc.70432
Cited by 2
AI Summary: OriGene’s pCMV6-AC-GFP lentiviral backbone expressing TurboGFP (catalog #PS100010) was used to generate constructs for expressing full-length PPARα in astrocytes, enabling the study of L-leucine’s effects on lysosomal biogenesis and autophagy in the context of Alzheimer’s disease.
Snippet: /label>DNA Constructs and Lentiviral TransductionsGeneration of the pCMV6-AC-GFP lentiviral backbone expressing TurboGFP (OriGene #PS100010) and FL-PPARα or
FigureFigureFigure
Showing 1-6 of 163 papers.
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