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pLenti-C-Myc-DDK Lentiviral Gene Expression Vector

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SKU
PS100064
pLenti-C-Myc-DDK, Lenti vector with C-terminal Myc-DDK tag, 10ug
  $880.00
In Stock*

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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Expression Promoter CMV
Features

This Lenti vector is a third generation of lentiviral vector.

  • All OriGene’s TrueORF clone can be shuttled into this vector via PrecisionShuttling, a simple cut-and-ligation process.
  • The ORF cloned in this vector can be expressed as a tagged protein with c-terminal Myc-DDK tags
  • Lentiviral infection is a convenient way to delivery DNA into most cell lines and is uniquely effective for the non-dividing cells.
  • A brief lentiviral packaging protocol is provided.
  • V2_F Primer can be used to sequence the target sequence after cloning.
  • LR50 Primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
Resources

file-alt Citations

Organoid platinum-resistance model identifies KRT17 as a biomarker of targeted therapy in ovarian cancer
Reichenbach, Schmid, Hierlmayer et al
iScience (2025) 28 (12), 113999-113999 DOI: 10.1016/j.isci.2025.113999
Cited by 2
AI Summary: OriGene products, specifically the Cytokeratin 17 (KRT17) Human Tagged Lenti ORF Clone (Cat# RC201619L1V) and the pLenti-C-Myc-DDK Lentiviral Gene Expression Vector (Cat# PS100064), were used to generate and express KRT17 in ovarian cancer organoid models to study its role as a biomarker of targeted therapy resistance.
Snippet: Cytokeratin 17 (KRT17) Human Tagged Lenti ORF Clone OriGene Cat#RC201619L1V pLenti-C-Myc-DDK Lentiviral Gene Expression Vector Origene Cat#SKU PS100064 Biological samples
Efficacy of futibatinib, an irreversible fibroblast growth factor receptor inhibitor, in FGFR-altered breast cancer
Saridogan, Akcakanat, Zhao et al
Sci Rep (2023) 13 (1), 20223 DOI: 10.1038/s41598-023-46586-y
Cited by 16
AI Summary: OriGene’s empty vector (#PS100064) was used as a control in viral expression experiments to study the effects of FGFR2 alterations and futibatinib sensitivity in breast cancer cell models.
Snippet: 5C mutation were created as described previously20. MCF10A and H69 cells were infected with virus packaged from HEK 293 T cells using viral expression vectors as well as an empty vector (#PS100064) (OriGene). Futibatinib was provided by Taiho Oncology Inc. AZD4547 and erdafitinib were purchased from Medkoo Biosciences, Inc. Dovitinib, infigratinib and lucitanib were purchased from Selleck Chemica
FigureFigureFigure
Topoisomerase Inhibitors Increase Episomal DNA Expression by Inducing the Integration of Episomal DNA in Hepatic Cells
Gómez-Moreno, San Sebastian, Moya et al
Pharmaceutics (2023) 15 (10) DOI: 10.3390/pharmaceutics15102459
Cited by 1
AI Summary: OriGene’s pLenti-C-Myc-DDK plasmid (cat. nº: PS100064) was used as the backbone for cloning the mCherry reporter gene, enabling the generation of reporter constructs essential for assessing the effects of topoisomerase inhibitors on viral gene expression and integration in hepatic cells.
Snippet: ions were prepared in DMSO and stored frozen at −20 °C until they were used in the experiments at the concentrations indicated in each experiment. The pLenti-C-Myc-DDK plasmid (cat. nº: PS100064, Origene, Rockville, MD, USA) was used for the cloning of the mCherry reporter gene. The lentivirus packaging vectors (pRSV-Rev, pMDLg-pRRE and pMD2.G) were obtained from the Addgene repository (Addgen
FigureFigureFigure
Boosting NAD preferentially blunts Th17 inflammation via arginine biosynthesis and redox control in healthy and psoriasis subjects
Han, Singh, Meadows et al
Cell Rep Med (2023) 4 (9), 101157 DOI: 10.1016/j.xcrm.2023.101157
Cited by 27
AI Summary: OriGene products pLenti-c-Myc-DDK, pLenti-c-Myc-DDK-NRF2, and pLenti-c-Myc-DDK-SQSTM1 were used in this study to manipulate gene expression in CD4+ T cells, enabling investigation of the roles of NRF2 and SQSTM1 in NAD+ boosting and Th17 immune responses.
Snippet: per;See Table S5 for details ------------------------- Bacterial and virus strains ------------------------- One Shot TOP10E. coli ThermoFisher Scientific Cat. C404003 pLenti-c-Myc-DDK Origene Technologies Cat. PS100064 pLenti-c-Myc-DDK-NRF2 Origene Technologies Cat. RC204140L2 pLenti-c-Myc-DDK-SQSTM1 Origene Technologies Cat. RC203214L1 ------------------------- Bio
FAM46C Is an Interferon-Stimulated Gene That Inhibits Lentiviral Particle Production by Modulating Autophagy
Mancino, Lai, De Grossi et al
Microbiol Spectr (2023)
Cited by 6
AI Summary: OriGene products PCMV6-Entry FAM46C plasmid (RC215475) and pLenti-C-Myc-DDK expression vector (PS100064) were used to generate FAM46C expression constructs, enabling the study of FAM46C’s role in inhibiting lentiviral particle production.
Snippet: … We recently showed that in MM cells FAM46C triggers apoptosis Save;The human FAM46C gene was subcloned from the nonlentiviral PCMV6-Entry FAM46C plasmid (Myc-DDK-tagged;Origene, RC215475) to the pLenti-C-Myc-DDK expression vector (Origene, PS100064) through insertion in the Sgf1 and MluI sites, giving rise to the pLenti-FAM46C-C-Myc-DDK plasmid. For doxycycline-inducible plasmids, the full-length FAM46C sequence was PCR amplified from the PCMV6-Myc-DDK FAM46C plasmid (Origene, RC215475)
Efficacy of futibatinib, an irreversible fibroblast growth factor receptor inhibitor, in FGFR-altered breast cancer
Meric-Bernstam, Saridogan, Akcakanat et al
Research Square (2022)
Snippet: Lentiviral constructs expressing FGFR2 WT, the FGFR2-BICC1 fusion, or the FGFR2 Y375C mutation were created as described previously 19 . MCF10A cells were infected with virus packaged from HEK 293T cells using viral expression vectors as well as an empty vector (#PS100064) (OriGene). F
Showing 1-6 of 20 papers.
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