Germinal Center B cell

Siva1 Mouse Gene Knockout Kit (CRISPR)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD45 (PTPRC) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800180) (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody. (UM800009; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD40 (RC201977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD40. Positive lysates LY400500 (100ug) and LC400500 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PTPRC (Cat# RC216590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRC (Cat# TA506567). Positive lysates LY401003 (100ug) and LC401003 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800145) (1:600)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD19 (RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19. Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD40 (Cat# RC201977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD40(Cat# TA807569). Positive lysates LY400500 (100ug) and LC400500 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Circular map for RC201242

Circular map for RC200410

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD19 (Cat# RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19(Cat# TA506236). Positive lysates LY400678 (100ug) and LC400678 (20ug) can be purchased separately from OriGene.

Circular map for RC202922

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human CD20 plasmid (RC201242, hCD20), mouse CD20 plasmid (MR203990, mCD20) using anti-CD20 antibody TA800385 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

Coomassie blue staining of purified CD19 protein (Cat# TP302922). The protein was produced from HEK293T cells transfected with CD19 cDNA clone (Cat# RC202922) using MegaTran 2.0 (Cat# TT210002).

Circular map for RC204252

Circular map for RC205302

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT20 mouse monoclonal antibody. (UM800065; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Circular map for RC203179

Coomassie blue staining of purified MS4A1 protein (Cat# TP301242). The protein was produced from HEK293T cells transfected with MS4A1 cDNA clone (Cat# RC201242) using MegaTran 2.0 (Cat# TT210002).

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-KRT20 mouse monoclonal antibody. (UM800060; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTPRC monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human SIVA1 plasmid (RC215680, hSIVA1), mouse SIVA1 plasmid (MR201528, mSIVA1) using anti-SIVA1 antibody TA506830 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human CD20 plasmid (RC201242, hCD20), mouse CD20 plasmid (MR203990, mCD20) using anti-CD20 antibody TA800533 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Circular map for RC201977

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PTPRC (Cat# RC216590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPRC (Cat# TA506567). Positive lysates LY401003 (100ug) and LC401003 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded human spleen using anti-CD20 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800002 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The spleen shows strong membrane and cytoplasmic staining in red and white pulp cells.

Circular map for RC216590

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD40 (RC201977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD40. Positive lysates LY400500 (100ug) and LC400500 (20ug) can be purchased separately from OriGene.

Circular map for RC211856

Circular map for RC215680

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD40 (Cat# RC201977, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD40(Cat# TA807569). Positive lysates LY400500 (100ug) and LC400500 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIVA1 (RC215680, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIVA1. Positive lysates LY416652 (100ug) and LC416652 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded human spleen using anti-CD20 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800002 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The spleen shows strong membrane and cytoplasmic staining in red and white pulp cells.

The binding affinity values of IgG and DuetMab with amino acid residue substitutions of the heavy chain and kappa light chain variable domains and their combinations of some of the substitutions to CD4 and CD70 (OriGene TP300410), as measured using an Octet384 instrument. Figure cited from MAbs, PMID: 25730144

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD38 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800153) (1:300)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD38 (Cat# RC203179, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD38 (Cat# TA804538)(1:2000). Positive lysates LY419752 (100ug) and LC419752 (20ug) can be purchased separately from OriGene.

RC204646L3 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC204646L3V particle to overexpress human MS4A4A-Myc-DDK fusion protein.

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-KRT20 mouse monoclonal antibody. (UM800060; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM800145) (1:600)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-CD40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, UM800075) (1:200)

Circular map for RC210417