HistoWiz Validated

Figure from citation: Positive correlation between LSD1, c-Myc, β-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, β-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100 View Citation. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Simple Western™ analysis of endogenous protein KRAS from HELA lysates (0.5 mg/mL) using KRAS Mouse Monoclonal Antibody #TA801672. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded testis using anti-MAGE-A4 C/N TA505362 clone OTI1F9 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, anti-MAGE-A4 diluted to 1:50. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image 40x magnification.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD1C (Cat# RC218490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD1C(Cat# TA505411). Positive lysates LY419762 (100ug) and LC419762 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD4 mouse monoclonal antibody. (UM800010; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD68 mouse monoclonal antibody. (UM800047; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human CD163 plasmid (RC208557, hCD163), mouse CD163 plasmid (MR219212, mCD163), rat CD163 plasmid (RR206586, rCD163) using anti-CD163 antibody TA506380 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD45 (PTPRC) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800180) (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD1C (Cat# RC218490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD1C(Cat# TA505411). Positive lysates LY419762 (100ug) and LC419762 (20ug) can be purchased separately from OriGene.

Figure from citation: Positive correlation between LSD1, c-Myc, β-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, β-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100 View Citation. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Figure from citation: Positive correlation between LSD1, c-Myc, β-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, β-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100 View Citation. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Simple Western™ analysis of endogenous protein KRAS from HELA lysates (0.5 mg/mL) using KRAS Mouse Monoclonal Antibody #TA801672. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD4 mouse monoclonal antibody. (UM800010; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded testis using anti-MAGE-A4 C/N TA505362 clone OTI1F9 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, anti-MAGE-A4 diluted to 1:50. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MKI67 mouse monoclonal antibody. (UM800033; heat-induced epitope retrieval by 10mM citric buffer, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD68 mouse monoclonal antibody. (UM800047; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD4 mouse monoclonal antibody. (UM800010; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Simple Western™ analysis of endogenous protein KRAS from HELA lysates (0.5 mg/mL) using KRAS Mouse Monoclonal Antibody #TA801672. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD1C (Cat# RC218490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD1C(Cat# TA505411). Positive lysates LY419762 (100ug) and LC419762 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD68 mouse monoclonal antibody. (UM800047; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded testis using anti-MAGE-A4 C/N TA505362 clone OTI1F9 mouse monoclonal antibody. HIER TEE buffer pH9 (B21-100) at 110C for 10 min, anti-MAGE-A4 diluted to 1:50. Detection was done with Polink1 Broad Mouse and Rabbit C/N D11-18 with DAB Kit. Image 40x magnification.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD45 (PTPRC) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800180) (1:500)

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human CD163 plasmid (RC208557, hCD163), mouse CD163 plasmid (MR219212, mCD163), rat CD163 plasmid (RR206586, rCD163) using anti-CD163 antibody TA506380 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human CD163 plasmid (RC208557, hCD163), mouse CD163 plasmid (MR219212, mCD163), rat CD163 plasmid (RR206586, rCD163) using anti-CD163 antibody TA506380 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD45 (PTPRC) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800180) (1:500)