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p53 (TP53) (NM_000546) Human Over-expression Lysate

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SKU
LY400186
Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 1 (as WB positive control)
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200003
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_000546, NP_000537
Synonyms BCC7; BMFS5; LFS1; P53; TRP53
Predicted MW 43.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Bioactivity TP53 Activity Verified in a DNA-binding Assay
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_000537
Locus ID 7157
Cytogenetics 17p13.1
Protein Families WB
Protein Pathways Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway
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Other "p53" proteins (16)
SKU Description Size Price
PH300003 TP53 MS Standard C13 and N15-labeled recombinant protein (NP_000537) 10 ug
$3,360.00
PH325502 TP53 MS Standard C13 and N15-labeled recombinant protein (NP_001119586) 10 ug
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PH325604 TP53 MS Standard C13 and N15-labeled recombinant protein (NP_001119584) 10 ug
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LC400186 Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426652 Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426653 Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426654 Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY426652 Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY426653 Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY426654 Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP300003 Recombinant protein of human tumor protein p53 (TP53), transcript variant 1, 20 µg 20 ug
$867.00
TP325502 Purified recombinant protein of Homo sapiens tumor protein p53 (TP53), transcript variant 3, 20 µg 20 ug
$867.00
TP325604 Recombinant protein of human tumor protein p53 (TP53), transcript variant 2, 20 µg 20 ug
$867.00
TP701001 Purified recombinant protein of human tumor protein P53 (TP53), transcript variant 1, mutant (Y220C), expressed in HEK293 cells, 20ug 20 ug
$900.00
TP710022 Recombinant protein of human tumor protein p53 (TP53), transcript variant 1, full length, with C-terminal DDK tag, expressed in sf9 cells. 20 ug
$540.00
TP750119 Purified recombinant protein of Human tumor protein p53 (TP53), transcript variant 1, full length, N-terminal His and GST tag, expressed in E. coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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