p53 (TP53) (NM_001126112) Human Over-expression Lysate
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC225604 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001126112, NP_001119584 |
| Synonyms | BCC7; BMFS5; LFS1; P53; TRP53 |
| Predicted MW | 43.5 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_001119584 |
| Locus ID | 7157 |
| Cytogenetics | 17p13.1 |
| Protein Families | WB |
| Protein Pathways | Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "p53" proteins (16)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH300003 | TP53 MS Standard C13 and N15-labeled recombinant protein (NP_000537) | 10 ug |
$3,360.00
|
|
| PH325502 | TP53 MS Standard C13 and N15-labeled recombinant protein (NP_001119586) | 10 ug |
$3,360.00
|
|
| PH325604 | TP53 MS Standard C13 and N15-labeled recombinant protein (NP_001119584) | 10 ug |
$3,360.00
|
|
| LC400186 | Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC426653 | Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC426654 | Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY400186 | Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| LY426652 | Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| LY426653 | Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 4 (as WB positive control) | 100 ug |
$436.00
|
|
| LY426654 | Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 3 (as WB positive control) | 100 ug |
$436.00
|
|
| TP300003 | Recombinant protein of human tumor protein p53 (TP53), transcript variant 1, 20 µg | 20 ug |
$867.00
|
|
| TP325502 | Purified recombinant protein of Homo sapiens tumor protein p53 (TP53), transcript variant 3, 20 µg | 20 ug |
$867.00
|
|
| TP325604 | Recombinant protein of human tumor protein p53 (TP53), transcript variant 2, 20 µg | 20 ug |
$867.00
|
|
| TP701001 | Purified recombinant protein of human tumor protein P53 (TP53), transcript variant 1, mutant (Y220C), expressed in HEK293 cells, 20ug | 20 ug |
$900.00
|
|
| TP710022 | Recombinant protein of human tumor protein p53 (TP53), transcript variant 1, full length, with C-terminal DDK tag, expressed in sf9 cells. | 20 ug |
$540.00
|
|
| TP750119 | Purified recombinant protein of Human tumor protein p53 (TP53), transcript variant 1, full length, N-terminal His and GST tag, expressed in E. coli, 50ug | 50 ug |
$270.00
|
|
Citations
Akt inhibitor augments anti-proliferative efficacy of a dual mTORC1/2 inhibitor by FOXO3a activation in p53 mutated hepatocarcinoma cells
Patra, Meyer, Ray et al
Cell Death Dis (2021) 12 (11), 1073 DOI: 10.1038/s41419-021-04371-7
Patra, Meyer, Ray et al
Cell Death Dis (2021) 12 (11), 1073 DOI: 10.1038/s41419-021-04371-7
Cited by 18
AI Summary: OriGene’s pcDNA3-p53 wild-type plasmid was used for transient transfection to study the effects of p53 restoration in Huh7 hepatocarcinoma cells.
Snippet: Huh7 cells were plated on 35-mm culture plate and transfected with a plasmid expressing pcDNA3-p53 wild-type (Origene, MD) or empty vector construct using Lipofectamine 2000 following the manufacturer's instruction (Life Tech, IL) for transient transfection and
A combination of AZD5363 and FH5363 induces lethal autophagy in transformed hepatocytes
Patra, Meyer, Ray et al
Cell Death Dis (2020) 11 (7), 540 DOI: 10.1038/s41419-020-02741-1
Patra, Meyer, Ray et al
Cell Death Dis (2020) 11 (7), 540 DOI: 10.1038/s41419-020-02741-1
Cited by 23
AI Summary: OriGene’s mCherry-EGFP-LC3 reporter construct was used in this study to visualize and assess autophagic flux in transformed human hepatocytes following treatment with AZD5363 and FH535, helping to demonstrate enhanced autophagy associated with the drug combination.
Inhibition of DYRK1A-EGFR axis by p53-MDM2 cascade mediates the induction of cellular senescence
Xu, Liu, Zhang et al
Cell Death Dis (2019) 10 (4), 282 DOI: 10.1038/s41419-019-1521-5
Xu, Liu, Zhang et al
Cell Death Dis (2019) 10 (4), 282 DOI: 10.1038/s41419-019-1521-5
AI Summary: In this study, the OriGene TP53 (p53) expression vector was used to investigate the role of p53 activation in regulating EGFR and DYRK1A levels, thereby contributing to the elucidation of the p53-MDM2-DYRK1A-EGFR signaling axis in cellular senescence.
Snippet: …Expression vector for p53 (TP53) was purchased from OriGene (Beijing, China). …Expression vector for p53 (TP53) was purchased from OriGene (Beijing, China). …
Showing 1-3 of 3 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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