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p53 (TP53) (NM_000546) Human Recombinant Protein

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SKU
TP300003
Recombinant protein of human tumor protein p53 (TP53), transcript variant 1, 20 µg
Size:
20 ug 1 mg 100 ug
  $867.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence
Protein Sequence
>Peptide sequence encoded by RC200003
Blue=ORF Red=Cloning site Green=Tag(s)

MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEA
APPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLA
KTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLD
DRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCA
CPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELN
EALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

Recombinant protein using RC200003 also available, TP300003
Tag C-Myc/DDK
Predicted MW 43.5 kDa
Concentration >0.1 µg/µL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Bioactivity TP53 Activity Verified in a DNA-binding Assay: TP53 (TP304649) activity was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the p53 consensus DNA-binding sequence was incubated with dilutions of the purified TP53 protein and TP53 bound to the oligo was captured onto the surface of a microtiter plate. After washing, bound TP53 was detected with an anti-p53 primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm.
EMSA reaction (PMID: 27183959)
Pull-down assay (PMID: 27515399)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Note For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Shipping Dry Ice
Reference Data
RefSeq NP_000537
Locus ID 7157
UniProt ID P04637
Cytogenetics 17p13.1
RefSeq Size 2591
RefSeq ORF 1179
Synonyms BCC7; BMFS5; LFS1; P53; TRP53
Summary This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). provided by RefSeq, Dec 2016
Protein Categories ADC Targets, Cancer: Bladder, Cancer: Brain and CNS, Cancer: Endocrine, Cancer: Gastrointestinal, Cancer: Gynecologic, Cancer: Haematopoietic and Lymphoid, Cancer: Head and Neck, Cancer: Lung, Cancer: Sarcoma, Cancer: Skin, Cardiovascular diseases, Growth Factors, Intracellular Proteins, Nervous system Diseases, Transciption Factors
Protein Families Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway
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Other "p53" proteins (16)
SKU Description Size Price
PH300003 TP53 MS Standard C13 and N15-labeled recombinant protein (NP_000537) 10 ug
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PH325502 TP53 MS Standard C13 and N15-labeled recombinant protein (NP_001119586) 10 ug
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PH325604 TP53 MS Standard C13 and N15-labeled recombinant protein (NP_001119584) 10 ug
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LC400186 Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426652 Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426653 Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC426654 Lyophilized TP53 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY400186 Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY426652 Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 2 (as WB positive control) 100 ug
$436.00
LY426653 Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 4 (as WB positive control) 100 ug
$436.00
LY426654 Transient overexpression lysate of tumor protein p53 (TP53), transcript variant 3 (as WB positive control) 100 ug
$436.00
TP325502 Purified recombinant protein of Homo sapiens tumor protein p53 (TP53), transcript variant 3, 20 µg 20 ug
$867.00
TP325604 Recombinant protein of human tumor protein p53 (TP53), transcript variant 2, 20 µg 20 ug
$867.00
TP701001 Purified recombinant protein of human tumor protein P53 (TP53), transcript variant 1, mutant (Y220C), expressed in HEK293 cells, 20ug 20 ug
$900.00
TP710022 Recombinant protein of human tumor protein p53 (TP53), transcript variant 1, full length, with C-terminal DDK tag, expressed in sf9 cells. 20 ug
$540.00
TP750119 Purified recombinant protein of Human tumor protein p53 (TP53), transcript variant 1, full length, N-terminal His and GST tag, expressed in E. coli, 50ug 50 ug
$270.00

file-alt Citations

Fortilin interacts with TGF-β1 and prevents TGF-β receptor activation
Pinkaew, Martinez-Hackert, Jia et al
Commun Biol (2022) 5 (1), 157 DOI: 10.1038/s42003-022-03112-6
Cited by 16
AI Summary: OriGene products, specifically recombinant human (rh) flag-tagged fortilin (Catalog #: TP301664) and rh-flag-tagged p53 (Catalog #: TP300003), were used in co-immunoprecipitation and western blot assays to investigate the interaction between fortilin and TGF-β1 in this study.
Snippet: stern blot analyses. Co-IP-western blot analysis Co-IP of TGF-β1 by fortilin: To evaluate whether fortilin specifically interacts with TGF-β1, we added recombinant human (rh) flag-tagged fortilin (OriGene, Rockville, MD, USA;Catalog #: TP301664), rh-flag-tagged p53 (OriGene, Catalog #: TP300003), rh-His6-tagged NQO2 (Abcam, Catalog #: ab93933), and rh-TGF-β1 (eBioscience, Catalog #: 14-8348-6
mTOR kinase leads to PTEN-loss-induced cellular senescence by phosphorylating p53
Jung, Hwang, Kang et al
Oncogene (2018) 38 (10), 1639-1650 DOI: 10.1038/s41388-018-0521-8
AI Summary: In this study, OriGene products—specifically the PTEN shRNA (OriGene, Cat# TL313805) and p53 antibody (OriGene, Cat# TA500249)—were used to manipulate PTEN expression and detect p53 protein, respectively, enabling the authors to investigate the molecular mechanisms underlying PTEN-loss-induced cellular senescence.
Snippet: …In this study, we show that mTOR acts as a critical relay molecule downstream of PI3K/AKT and upstream of p53 in PICS. We found that PTEN depletion induces cellular senescence via p53-p21 signaling without triggering DNA damage response. mTOR kinase, a major component of mTORC1 and mTORC2, directly binds p53 and phosphorylates it at serine 15. mTORC1 and mTORC2 compete with MDM2 and increase the stability of p53 to induce cellular senescence via accumulation of the cell cycle inhibitor, p21. In embryonic fibroblasts of PTEN-knockout mice, PTEN deficiency also induces mTORC1 and mTORC2 to bind to p53 instead of MDM2, leading to cellular senescence. These results collectively demonstrate for the first time that mTOR plays a critical role in switching cells from proliferation signaling to senescence signaling via a direct link between the growth-promoting activity of AKT and the growth-suppressing activity of p53
Analysis of binding interfaces of the human scaffold protein AXIN1 by peptide microarrays
Harnoš, Ryneš, Víšková et al
J. Biol. Chem. (2018) 293 (42), 16337-16347 DOI: 10.1074/jbc.RA118.005127
AI Summary: In this study, OriGene products CK1ε (#TP302436), p53 (#TP300003), and Pin1 (#TP302543) were used as purified proteins to investigate and validate their binding interactions with AXIN1 using peptide microarrays.
Snippet: CK1ε (OriGene Technologies' #TP302436), p53 (OriGene Technologies, #TP300003) Pin1 (OriGene Technologies #TP302543), c-Myc (Abnova, #H00004609-P01) in SmartBlock FLAG-DVL3 wt(30), CK1ϵ wt(17), Constitutive active β-catenin S33A(31), FLAG-Shp2
A Novel In Vitro CypD-Mediated p53 Aggregation Assay Suggests a Model for Mitochondrial Permeability Transition by Chaperone Systems
Lebedev, Nemajerova, Foda et al
J. Mol. Biol. (2016)
Cited by 48
AI Summary: OriGene contributed purified wild-type full-length human p53 protein (Cat # TP300003), expressed in HEK293 cells and used in the study's in vitro aggregation assays to investigate p53's role in mitochondrial permeability transition.
Snippet: Purified WT full-length human p53 was purchased from OriGene (Cat # TP300003). The protein was expressed in HEK293 cells as a C-terminal MYC/DDK fusion protein and affinity purified
Species-specific mutual regulation of p53 and miR-138 between human, rat and mouse
Li, Xia, Su et al
Sci Rep (2016) 6, 26187 DOI: 10.1038/srep26187
Cited by 13
AI Summary: OriGene products, including recombinant p53 protein and pCMV/p63 and pCMV/p73 plasmids, were used in this study to investigate the species-specific regulatory interactions between p53 and miR-138.
Snippet: Primer sequences used for ChIP are listed in Table S1. EMSA. Recombinant p53 protein was purchased from OriGene (OriGene Technologies, Rockville, MD, USA). ... pCMV/p63 and pCMV/p73 were all purchased from OriGene (OriGene Technologies, Rockville, MD ... Cite SaveSaving
Dynamics of re-constitution of the human nuclear proteome after cell division is regulated by NLS-adjacent phosphorylation
Róna, Borsos, Ellis et al
Cell Cycle (2014) 13 (22), 3551-64 DOI: 10.4161/15384101.2014.960740
Cited by 25
AI Summary: In this study, OriGene products such as the human dUTPase cDNA clone (RC200475) and dUTPase-specific antibodies (TA500482) were utilized to express and detect dUTPase, enabling the investigation of how NLS-adjacent phosphorylation regulates its nuclear import during the cell cycle.
Showing 1-6 of 7 papers.
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