p53 (TP53) (NM_000546) Human Recombinant Protein

CAT#: TP300003

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Recombinant protein of human tumor protein p53 (TP53), transcript variant 1


 Product Datasheet for 'TP300003'

 Cited in 4 publications.

  View other "TP53" proteins (18)

USD 748.00


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    • 20 ug


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Specifications

Product Data
Description Recombinant protein of human tumor protein p53 (TP53), transcript variant 1
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC200003. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag C-Myc/DDK
Predicted MW 43.5 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
Bioactivity TP53 Activity Verified in a DNA-binding Assay: TP53 (TP304649) activity was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the p53 consensus DNA-binding sequence was incubated with dilutions of the purified TP53 protein and TP53 bound to the oligo was captured onto the surface of a microtiter plate. After washing, bound TP53 was detected with an anti-p53 primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm.
EMSA reaction (PMID: 27183959)
Pull-down assay (PMID: 27515399)
Preparation Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Reference Data
RefSeq NP_000537
Locus ID 7157
Refseq Size 2591
Cytogenetics 17p13.1
Refseq ORF 1179
Synonyms BCC7; LFS1; P53; TRP53
Summary This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Protein Families Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways Amyotrophic lateral sclerosis (ALS), Apoptosis, Basal cell carcinoma, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Glioma, Huntington's disease, MAPK signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Wnt signaling pathway

Citations (4)

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BOGO Free lysates
68 Mouse Clones
HUMAN PROREINS 101