Mcam Mouse Gene Knockout Kit (CRISPR)

CAT#: KN309859BN

Mcam - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 mBFP-Neo donor, 1 scramble control
• Donor DNA: mBFP-Neo
• Symbol: Mcam
• Locus ID: 84004
• Components:

  KN309859G1, Mcam gRNA vector 1 in pCas-Guide CRISPR vector

  KN309859G2, Mcam gRNA vector 2 in pCas-Guide CRISPR vector

  KN309859BND, donor DNA containing left and right homologous arms and mBFP-Neo functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_023061, NM_001359530
• UniProt ID: Q8R2Y2
• Synonyms: 1-gicerin; AV025631; CD146; CD149; Muc18; s-endo; s-gicerin
• Summary: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).[UniProtKB/Swiss-Prot Function]