MRP5 (ABCC5) Human Gene Knockout Kit (CRISPR)

SKU KN217669RB

ABCC5 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Vector: RFP-BSD
• Target Symbol: MRP5
• Locus ID: 10057
• Components:

  KN217669G1, MRP5 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GTCCTGGGTATAGAAGTGTG

  KN217669G2, MRP5 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TCTTAGGAAAGGAAACTGAC

  KN217669RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  Homologous arm and RFP-BSD sequences:

  pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

  TTAACTGGAT AACTTCATGG AGCTTTAAAA TAACACCAGC TAACCCATTA GTTAATTATA AAAAGGCAGA GATGGAATAT ATCATTGGTA GAATAAGTGT TTGGTAAGTT TTTATTTAGT AAAATTTAGT AGAAGAAATG ATGTCCACTT TAAAATTGTG GAGTTTTTAA TACTTGGGAC AAACTGAAAT TGTTTCTAGA AAGTCTTTAA AAAAAAAACC AACCTTTCTA TTGTATACAC TGGATTTGAG CTTAGTTGAA GAGAATGGGT GTCTCTTTAC TAGTGTGTTT ACTGTTTCTA ATTTTTTTTC CCTTAGAGTT GGGAGAAGGT AATTTTTCTT GATTCTCTTA TACTGAATCT TTTTCTTTAA AAAAATGAAA TCAAAATCTT TGAACTTTTT TTGAAAATTA ATTTTTGTAG CTATAACAGT AGAAAGCTTT AGTACTTTTG CCTTTTTTTT TTTTTTCCTA TTTTTAAGGC ACTTGAGCTT ATGGTTAAGT ACTGTTTAGG AAATAATCTA TTTCTTGATG AATTTTTAAT TTCAGGAATT CTGATGTGAA ACTAACAGTC TGTGAGCCCT GGAACCTCCA CTCAGAGAAG GTTGTACTAA AACACATCTT TTTTCGTATA ATGGGTAATT TAGAAACTTA TTTTTTGGCC GGGCGCGGTG GCTCACGCCT ATAAACCCAG CACTTTGGGA GGCTGAGGCG GGTGGATCAT CTGAGGTCAG GAGTTCGAGA CCAGCCTGAC CAACATGGTG AAAACCTGTC TCTACTAAAA GTACAAAAAT TAGCCAGGCG TGGTGGCGGG CGCCTATAAT CCCAGATACT TGCGAGGCTG AGGCACGAGA ATCGCTTGAA CCCAAGAGGC GGAGGTTGCA GTGAGCTGGG ATCATGCCAC TGCACTCCAG CCTGGGCGAT AGAGCGAGAC TCAGTCTCAA AAAAAAATGT GTTTTTCTTT ATTGGCTTTA GGTTACTGTT AAGAATTTAT GGTTATTGAT AAGTCTCTGG TCCATAGTAA GCACTCAATA AGTGGAAGAG AGGCTGGCTG TCACCCTCTC ACCCGGTCTG TTCTGAGCAT TCTCCTCTTT TTCACTTTTA TTCTATGTTG GTGCCTTCTG AAAAGGCTAT ACTTAACAGA AGACAACTTA TTGAGGGGGA GGGGGTCATG GGGTCAATGA CAGGGAATAT AAAGCCAATT

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001023587, NM_001320032, NM_005688, NR_135125
• UniProt ID: O15440
• Synonyms: ABC33; EST277145; MOAT-C; MOATC; MRP5; pABC11; SMRP
• Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
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