Neuroscience

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TGM2 (Cat# RC217353, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGM2 (Cat# TA809245). Positive lysates LY401458 (100ug) and LC401458 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLTA (RC203505, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLTA (1:2000). Positive lysates LY400696 (100ug) and LC400696 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAX (RC204369, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with Rabbit polycl. Positive lysates LY408504 (100ug) and LC408504 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein GAPDH from HEK293T lysates (0.2 mg/mL) using GAPDH Mouse Monoclonal Antibody #TA802519. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human IL1B plasmid (RC202079, hIL1B), mouse IL1B plasmid (MR226719, mIL1B), rat IL1B plasmid (RR211023, rIL1B) using anti-IL1B antibody TA506443 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TP53 mouse monoclonal antibody. (UM500053; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Simple Western™ analysis of endogenous protein GAPDH from HEK293T lysates (0.2 mg/mL) using GAPDH Mouse Monoclonal Antibody #TA802519. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Simple Western™ analysis of endogenous protein GAPDH from HEK293T lysates (0.2 mg/mL) using GAPDH Mouse Monoclonal Antibody #TA802519. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human Prolactin plasmid (RC205627, hProlactin), mouse Prolactin plasmid (MR227036, mProlactin) using anti-Prolactin antibody TA500719 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human TP53 plasmid (RC200003, hTP53), mouse TP53 plasmid (MR206086, mTP53), rat TP53 plasmid (RR201296, rTP53) using anti-TP53 antibody TA502870 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human VMAT2 plasmid (RC221342, hVMAT2) using anti-VMAT2 antibody TA500506 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAPK1 (Cat# RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1 (Cat# TA500468). Positive lysates LY408481 (100ug) and LC408481 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB10 (RC200526, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB10 (TA505207). Positive lysates LY417917 (100ug) and LC417917 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BID mouse monoclonal antibody. (UM500101, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human NDUFB10 plasmid (RC200526, hNDUFB10), mouse NDUFB10 plasmid (MR201544, mNDUFB10) using anti-NDUFB10 antibody TA505113 (1:10000@1mg/ml). Figure B, Western blot analysis of the same human NDUFB10 and mouse NDUFB10 samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and UQCRC1-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-UQCRC1 monoclonal antibody TA800089(1:2000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

Simple Western™ analysis of endogenous protein SDHB from HEK293T lysates (0.2 mg/mL) using SDHB Mouse Monoclonal Antibody #TA808102. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY POLR2E (Cat# RC201266, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E (Cat# TA502548). Positive lysates LY419161 (100ug) and LC419161 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UBE2J1 (Cat# RC202826, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2J1 (Cat# TA504988). Positive lysates LY414230 (100ug) and LC414230 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HSD17B10 (Cat# RC201734, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B10(Cat# TA500686). Positive lysates LY401426 (100ug) and LC401426 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK1 (RC204703, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK1. Positive lysates LY408481 (100ug) and LC408481 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NDUFS2 (Cat# RC203485, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFS2 (Cat# TA802340). Positive lysates LY417919 (100ug) and LC417919 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LPL (Cat# RC203766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPL (Cat# TA503793). Positive lysates LY400089 (100ug) and LC400089 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MME (Cat# RC223013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MME (Cat# TA504507)(1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (RC201638, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B. Positive lysates LY400637 (100ug) and LC400637 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800128) (1:600)

Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human Prolactin plasmid (RC205627, hProlactin), mouse Prolactin plasmid (MR227036, mProlactin) using anti-Prolactin antibody TA500719 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CDK5 (Cat# RC200342, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK5 (Cat# TA500397). Positive lysates LY401536 (100ug) and LC401536 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NDUFB9 (Cat# RC200223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB9 (Cat# TA502568). Positive lysates LY417578 (100ug) and LC417578 (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human LPAR1 plasmid (RC206065, lane 2), mouse LPAR1 plasmid (MR205601, lane 3), rat LPAR1 plasmid (RR210163, lane 4) using anti-LPAR1 antibody TA503737 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAPDH (Cat# RC202309, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAPDH (Cat# TA802563). Positive lysates LY419567 (100ug) and LC419567 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SOD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-APP mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Simple Western™ analysis of endogenous protein GAPDH from HEK293T lysates (0.2 mg/mL) using GAPDH Mouse Monoclonal Antibody #TA802519. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human APP plasmid (RC221339, hAPP), mouse APP plasmid (MR210090, mAPP), rat APP plasmid (RR204173, rAPP) using anti-APP antibody TA500969 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MAPT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800131) (1:300)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-APP mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UQCRFS1 (Cat# RC204051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UQCRFS1 (1:2000) (Cat# TA811461). Positive lysates LY416927 (100ug) and LC416927 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LPL (Cat# RC203766, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LPL (Cat# TA503793). Positive lysates LY400089 (100ug) and LC400089 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY AP2M1 (Cat# RC201377, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AP2M1(Cat# TA503015). Positive lysates LY422484 (100ug) and LC422484 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MME (Cat# RC223013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MME (Cat# TA504507)(1:500).

LEP Antibody (N-term) (Cat. #AP52465PU-N) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemical staining of paraffin-embedded Human normal colon tissue and colonrectal cancer tissue using anti-TP53 mouse monoclonal antibody. (UM500049; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Simple Western™ analysis of endogenous protein NDUFB10 from HELA lysates (0.1 mg/mL) using NDUFB10 Mouse Monoclonal Antibody #TA505114. The virtual lane view (left) shows the target (as indicated) at a 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-UBE2J1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.