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CRISPRa SAM Vector Kit, CRISPR/Cas9 Gene Activation

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SKU
GE100057
CRISPRa SAM vector kit containing pCas-Guide-CRISPRa (GE100055), pCRISPRa-Enhancer (GE100056) and pCas-Guide-CRISPRa-Scramble (GE100058)
  $1,290.00
2 Weeks*

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TP790148 Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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Specifications
Specifications
Product Data
Function CRISPRa SAM vector kit
Features
  • Robust CRISPR gene activation system
  • Synergistic activation by three activation domains, VP64, p65 and HSF1
  • Two vector system, dCas9-VP64-gRNA(MS2) (SKU GE100055) and MS2-p65-HSF1 (SKU GE100056)
  • Scramble gRNA control (SKU GE100058) is included
  • CF3 primer can be used to sequence target sequence after cloning
  • Complement to gene overexpression with cDNA clones
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
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file-alt Citations

Ovotransferrin Exhibits Osteogenic Activity Partially via Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Activation in MC3T3-E1 Cells
Shang, Bhullar, Wu
J. Agric. Food Chem. (2020) 68 (35), 9427-9435 DOI: 10.1021/acs.jafc.0c04064
Cited by 17
AI Summary: OriGene products, specifically the LRP1 CRISPR/Cas9 KO kit (with LRP1 gRNA vectors and KO plasmid) and TurboFectin Transfection Reagent, were used to delete the LRP1 gene and facilitate transfection in MC3T3-E1 cells, enabling the study of LRP1’s role in ovotransferrin-induced osteogenic activity.
Snippet: LRP1 CRISPR/Cas9 KO kit with 2 LRP1 gRNA vectors was obtained 101 from OriGene Technologies, Inc. (Rockville, MD, USA) and a LRP1 CRISPR/Cas9 KO plasmid The TurboFectin Transfection 103 Reagent was obtained from OriGene Technologies, Inc
Rapid identification of human mast cell degranulation regulators using functional genomics coupled to high-resolution confocal microscopy
Folkerts, Gaudenzio, Maurer et al
Nat Protoc (2020) 15 (3), 1285-1310 DOI: 10.1038/s41596-019-0288-6
Cited by 34
AI Summary: OriGene products, specifically the HuSH-29 shRNA plasmids and the TrueORF cDNA clones, were used in this study to enable targeted gene knockdown and overexpression, facilitating the identification of regulators of human mast cell degranulation through functional genomics and high-resolution confocal microscopy.
FigureFigureFigure
Mesenchymal Stem Cell Engineering
Liu
Methods Mol. Biol. (2018) 1868, 145-150 DOI: 10.1007/978-1-4939-8802-0_15
Snippet: Open image in new window Fig. 1. Fig. 1 Scheme of genome editing via CRISPR/Cas9. (This figure is adapted from the CRISPR/Cas9 Gnome Editing Application Guide, Origene.) (1) CRISPR/Cas cuts the double-stranded DNA at the target site 3. TurboFectin 8.0
PGE2/EP3/SRC signaling induces EGFR nuclear translocation and growth through EGFR ligands release in lung adenocarcinoma cells
Bazzani, Donnini, Finetti et al
Oncotarget (2017) 8 (19), 31270-31287 DOI: 10.18632/oncotarget.16116
Snippet: Cells were transiently transfected with 1 μg PX458 using an Amaxa Nucleofector machine (Lonza, Basel, Switzerland) according to manufacturer's instructions. 48 h post transfection, GFP-expressing cells were FACS sorted and re-seeded at limiting dilution in 96-well plates in order to obtain individual clones. GLC82 EGFR knockout cells were generated using CRISPR/Cas9 human gene knockout kit (OriGen...
FigureFigureFigure
Showing 1-4 of 4 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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