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GATA3 (NM_001002295) Human Over-expression Lysate

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SKU
LY424146
Transient overexpression lysate of GATA binding protein 3 (GATA3), transcript variant 1 (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211904
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001002295, NP_001002295
Synonyms HDR; HDRS
Predicted MW 48 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Bioactivity DNA-binding activity
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001002295
Locus ID 2625
Cytogenetics 10p14
Protein Families WB
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Other "GATA3" proteins (8)
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LY400759 Transient overexpression lysate of GATA binding protein 3 (GATA3), transcript variant 2 (as WB positive control) 100 ug
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TP301618 Recombinant protein of human GATA binding protein 3 (GATA3), transcript variant 2, 20 µg 20 ug
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TP761574 Purified recombinant protein of Human GATA binding protein 3 (GATA3), transcript variant 1, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug 50 ug
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TP762348 Purified recombinant protein of Human GATA binding protein 3 (GATA3), transcript variant 1, Pro189-Ser201(8 reapeat regions linked with GGGGS), with N-terminal His tag, expressed in E.coli, 50ug 50 ug
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TP762449 Purified recombinant protein of Human GATA binding protein 3 (GATA3), transcript variant 1, Met1-Thr156, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
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file-alt Citations

Glutamine synthetase is a genetic determinant of cell type-specific glutamine independence in breast epithelia
Kung, Marks, Chi
PLoS Genet. (2011) 7 (8), e1002229 DOI: 10.1371/journal.pgen.1002229
Cited by 283
AI Summary: In this study, OriGene products—specifically the GATA3 cDNA clone (RC210307) and GS (GLUL) shRNA lentiviral particles (TL309976V)—were used to manipulate gene expression in breast epithelial cells, enabling the authors to elucidate the genetic mechanisms underlying cell type-specific glutamine dependence.
Snippet: When we examined the properties associated with the distinct need for glutamine, we found the cell lines that exhibit glutamine dependence are all of the basal-type whereas the four lines that are more glutamine independent are luminal-type cells (Figure 1A, 1B) [34].
FigureFigureFigure
Showing 1-1 of 1 papers.
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