GATA3 (NM_002051) Human Recombinant Protein
SKU
TP301618
Recombinant protein of human GATA binding protein 3 (GATA3), transcript variant 2, 20 µg
$867.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293 |
| Expression cDNA Clone or AA Sequence | |
| Tag | C-Myc/DDK |
| Predicted MW | 47.7 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Bioactivity | GATA3 Activity Verified in a DNA-binding Assay: DNA binding activity of GATA3 as a function of protein concentration. Sensitivity to competitor oligonucleotide demonstrates specificity of protein-DNA binding. EMSA assay (PMID: 26216189) |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
| RefSeq | NP_002042 |
| Locus ID | 2625 |
| UniProt ID | P23771 |
| Cytogenetics | 10p14 |
| RefSeq Size | 3067 |
| RefSeq ORF | 1332 |
| Synonyms | HDR; HDRS |
| Summary | This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. provided by RefSeq, Nov 2009 |
| Protein Categories | Allergies and autoimmune diseases, Cancer: Gynecologic, Cytokines, Growth Factors, Intracellular Proteins, Transciption Factors |
| Protein Families | Adult stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "GATA3" proteins (8)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH301618 | GATA3 MS Standard C13 and N15-labeled recombinant protein (NP_002042) | 10 ug |
$3,360.00
|
|
| LC400759 | Lyophilized GATA3 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC424146 | Lyophilized GATA3 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY400759 | Transient overexpression lysate of GATA binding protein 3 (GATA3), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| LY424146 | Transient overexpression lysate of GATA binding protein 3 (GATA3), transcript variant 1 (as WB positive control) | 100 ug |
$665.00
|
|
| TP761574 | Purified recombinant protein of Human GATA binding protein 3 (GATA3), transcript variant 1, full length, with N-terminal GST and C-terminal HIS tag, expressed in E. coli, 50ug | 50 ug |
$270.00
|
|
| TP762348 | Purified recombinant protein of Human GATA binding protein 3 (GATA3), transcript variant 1, Pro189-Ser201(8 reapeat regions linked with GGGGS), with N-terminal His tag, expressed in E.coli, 50ug | 50 ug |
$260.00
|
|
| TP762449 | Purified recombinant protein of Human GATA binding protein 3 (GATA3), transcript variant 1, Met1-Thr156, with N-terminal His tag, expressed in E.coli, 50ug | 50 ug |
$240.00
|
|
Citations
Gata3-controlled nucleosome eviction drives Myc enhancer activity in T-cell development and leukemia
Belver, Yang, Albero et al
Cancer Discov (2019) 9 (12), 1774-1791 DOI: 10.1158/2159-8290.CD-19-0471
Belver, Yang, Albero et al
Cancer Discov (2019) 9 (12), 1774-1791 DOI: 10.1158/2159-8290.CD-19-0471
AI Summary: OriGene’s GATA3 protein was used to incubate with nucleosome arrays, enabling the study of GATA3-driven nucleosome eviction and its role in modulating Myc enhancer activity during T-cell development and leukemia.
Snippet: We incubated extended and compacted nucleosome arrays with GATA3 (Origene) for two hours at room temperature in binding buffer containing 10mM Tris pH 7.5, 1mM βmercaptoethanol, 40mM KCl, 5mM DTT, 250ug/mL BSA, 1% ficoll and 5% glycerol. We digested nucleosome arrays with 40U/mL of DNAse I (NEB) for one minute at room temperature
Transactivation of human osteoprotegerin promoter by GATA-3
Kao, Stankovic
Sci Rep (2015) 5, 12479 DOI: 10.1038/srep12479
Kao, Stankovic
Sci Rep (2015) 5, 12479 DOI: 10.1038/srep12479
Cited by 2
AI Summary: In this study, purified human GATA-3 protein from OriGene was used in gel shift assays to investigate the binding of GATA-3 to regulatory elements in the human osteoprotegerin (OPG) promoter.
Snippet: Gel shift assay. The gel shift assay was performed using the LightShift TM EMSA Optimization &Control Kit (#20148, Thermo Scientific) according to the manufacturer's manual. The purified human GATA-3 protein was from OriGene. ... Cite SaveSaving
Showing 1-2 of 2 papers.
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