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GATA3 (NM_001002295) Human Over-expression Lysate

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SKU
LC424146
Lyophilized GATA3 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211904
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001002295, NP_001002295
Synonyms HDR; HDRS
Predicted MW 48 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001002295
Locus ID 2625
Cytogenetics 10p14
Protein Families WB
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file-alt Citations

Glutamine synthetase is a genetic determinant of cell type-specific glutamine independence in breast epithelia
Kung, Marks, Chi
PLoS Genet. (2011) 7 (8), e1002229 DOI: 10.1371/journal.pgen.1002229
Cited by 283
AI Summary: In this study, OriGene products—specifically the GATA3 cDNA clone (RC210307) and GS (GLUL) shRNA lentiviral particles (TL309976V)—were used to manipulate gene expression in breast epithelial cells, enabling the authors to elucidate the genetic mechanisms underlying cell type-specific glutamine dependence.
Snippet: When we examined the properties associated with the distinct need for glutamine, we found the cell lines that exhibit glutamine dependence are all of the basal-type whereas the four lines that are more glutamine independent are luminal-type cells (Figure 1A, 1B) [34].
FigureFigureFigure
Showing 1-1 of 1 papers.
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