CD8A (NM_001768) Human Over-expression Lysate

SKU
LY419749
Transient overexpression lysate of CD8a molecule (CD8A), transcript variant 1 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206608
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001768, NP_001759
Synonyms CD8; Leu2; p32
Predicted MW 25.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_001759
Locus ID 925
Cytogenetics 2p11.2
Protein Families WB
Protein Pathways Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway
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Other "CD8A" proteins (13)
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TP760215 Recombinant protein of human CD8a molecule (CD8A), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
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file-alt Citations

Slc4a11 disruption causes duct cell loss and impairs NaCl reabsorption in female mouse submandibular glands
Yang, Mukaibo, Gao et al
Physiol Rep (2019) 7 (23), e14232 DOI: 10.14814/phy2.14232
Cited by 9
AI Summary: OriGene products contributed to this study by providing plasmids encoding mouse Slc4a11, human CD8A, and the empty pCMV6 entry vector, which were used for transfection experiments to investigate the function of Slc4a11 in mouse submandibular glands.
Snippet: 2001). Plasmids encoding mouse Slc4a11 (GenBank accession no. NM_ 001081162), human CD8A (GenBank accession no. NM_001768) and the empty pCMV6 entry vector as a transfection control were obtained from OriGene (OriGene Technologies, Rockville
FigureFigureFigure
Gene-modified NK-92MI cells expressing a chimeric CD16-BB-ζ or CD64-BB-ζ receptor exhibit enhanced cancer-killing ability in combination with therapeutic antibody
Chen, You, Jiang et al
Oncotarget (2017) 8 (23), 37128-37139 DOI: 10.18632/oncotarget.16201
Snippet: The FcRγIIIa CD16 (158F) cDNA extracellular domain (NM_001127593), FcγRI (CD64) cDNA extracellular domain (AK291451), and the CD8a (NM_001768) extracellular domain were obtained from Origene and synthesized. …
FigureFigureFigure
Showing 1-2 of 2 papers.
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