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CD8A (NM_171827) Human Over-expression Lysate

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SKU
LY403530
Transient overexpression lysate of CD8a molecule (CD8A), transcript variant 2 (as WB positive control)
  $436.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213165
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_171827, NP_741969
Synonyms CD8; Leu2; p32
Predicted MW 19.4 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_741969
Locus ID 925
Cytogenetics 2p11.2
Protein Families WB
Protein Pathways Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway
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Other "CD8A" proteins (13)
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LC403530 Lyophilized CD8A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC429040 Lyophilized CD8A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY419749 Transient overexpression lysate of CD8a molecule (CD8A), transcript variant 1 (as WB positive control) 100 ug
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LY429040 Transient overexpression lysate of CD8a molecule (CD8A), transcript variant 3 (as WB positive control) 100 ug
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TP721308 Human CD8α Protein (C-His) 25 ug
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TP760215 Recombinant protein of human CD8a molecule (CD8A), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
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TP761244 Purified recombinant protein of Human CD8a molecule (CD8A), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
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TP761250 Purified recombinant protein of Human CD8a molecule (CD8A), transcript variant 3, full length, with N-terminal HIS tag, expressed in E. coli, 50ug 50 ug
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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