CD8A (NM_001768) Human Over-expression Lysate
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC206608 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_001768, NP_001759 |
| Synonyms | CD8; Leu2; p32 |
| Predicted MW | 25.7 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_001759 |
| Locus ID | 925 |
| Cytogenetics | 2p11.2 |
| Protein Families | WB |
| Protein Pathways | Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CD8A" proteins (13)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC403530 | Lyophilized CD8A HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LC429040 | Lyophilized CD8A HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY403530 | Transient overexpression lysate of CD8a molecule (CD8A), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| LY419749 | Transient overexpression lysate of CD8a molecule (CD8A), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| LY429040 | Transient overexpression lysate of CD8a molecule (CD8A), transcript variant 3 (as WB positive control) | 100 ug |
$436.00
|
|
| TP721308 | Human CD8α Protein (C-His) | 25 ug |
$300.00
|
|
| TP721309 | Human CD8α Protein (C-His-Avi) | 25 ug |
$300.00
|
|
| TP721310 | Biotinylated Human CD8α Protein (C-His-Avi) | 25 ug |
$430.00
|
|
| TP721311 | PE Conjugated Human CD8α Protein (C-His) | 25 ug |
$430.00
|
|
| TP721312 | APC Conjugated Human CD8α Protein (C-His) | 25 ug |
$430.00
|
|
| TP760215 | Recombinant protein of human CD8a molecule (CD8A), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | 50 ug |
$270.00
|
|
| TP761244 | Purified recombinant protein of Human CD8a molecule (CD8A), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug | 50 ug |
$270.00
|
|
| TP761250 | Purified recombinant protein of Human CD8a molecule (CD8A), transcript variant 3, full length, with N-terminal HIS tag, expressed in E. coli, 50ug | 50 ug |
$270.00
|
|
Citations
Slc4a11 disruption causes duct cell loss and impairs NaCl reabsorption in female mouse submandibular glands
Yang, Mukaibo, Gao et al
Physiol Rep (2019) 7 (23), e14232 DOI: 10.14814/phy2.14232
Yang, Mukaibo, Gao et al
Physiol Rep (2019) 7 (23), e14232 DOI: 10.14814/phy2.14232
Cited by 9
AI Summary: OriGene products contributed to this study by providing plasmids encoding mouse Slc4a11, human CD8A, and the empty pCMV6 entry vector, which were used for transfection experiments to investigate the function of Slc4a11 in mouse submandibular glands.
Snippet: 2001). Plasmids encoding mouse Slc4a11 (GenBank accession no. NM_ 001081162), human CD8A (GenBank accession no. NM_001768) and the empty pCMV6 entry vector as a transfection control were obtained from OriGene (OriGene Technologies, Rockville
Gene-modified NK-92MI cells expressing a chimeric CD16-BB-ζ or CD64-BB-ζ receptor exhibit enhanced cancer-killing ability in combination with therapeutic antibody
Chen, You, Jiang et al
Oncotarget (2017) 8 (23), 37128-37139 DOI: 10.18632/oncotarget.16201
Chen, You, Jiang et al
Oncotarget (2017) 8 (23), 37128-37139 DOI: 10.18632/oncotarget.16201
Snippet: The FcRγIIIa CD16 (158F) cDNA extracellular domain (NM_001127593), FcγRI (CD64) cDNA extracellular domain (AK291451), and the CD8a (NM_001768) extracellular domain were obtained from Origene and synthesized. …
Showing 1-2 of 2 papers.
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