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RGS3 (NM_134427) Human Over-expression Lysate

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SKU
LY408751
Transient overexpression lysate of regulator of G-protein signaling 3 (RGS3), transcript variant 4 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC206471
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_134427, NP_602299
Synonyms C2PA; RGP3
Predicted MW 100.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_602299
Locus ID 5998
Cytogenetics 9q32
Protein Families WB
Protein Pathways Axon guidance
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Other "RGS3" proteins (16)
SKU Description Size Price
PH306471 RGS3 MS Standard C13 and N15-labeled recombinant protein (NP_602299) 10 ug
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PH324058 RGS3 MS Standard C13 and N15-labeled recombinant protein (NP_570613) 10 ug
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PH324111 RGS3 MS Standard C13 and N15-labeled recombinant protein (NP_066929) 10 ug
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LC402835 Lyophilized RGS3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC403390 Lyophilized RGS3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC408751 Lyophilized RGS3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC408949 Lyophilized RGS3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC413532 Lyophilized RGS3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY402835 Transient overexpression lysate of regulator of G-protein signaling 3 (RGS3), transcript variant 2 (as WB positive control) 100 ug
$665.00
LY403390 Transient overexpression lysate of regulator of G-protein signaling 3 (RGS3), transcript variant 5 (as WB positive control) 100 ug
$436.00
LY408949 Transient overexpression lysate of regulator of G-protein signaling 3 (RGS3), transcript variant 1 (as WB positive control) 100 ug
$436.00
LY413532 Transient overexpression lysate of regulator of G-protein signaling 3 (RGS3), transcript variant 3 (as WB positive control) 100 ug
$665.00
TP306471 Recombinant protein of human regulator of G-protein signaling 3 (RGS3), transcript variant 4, 20 µg 20 ug
$737.00
TP324058 Recombinant protein of human regulator of G-protein signaling 3 (RGS3), transcript variant 1, 20 µg 20 ug
$737.00
TP324111 Recombinant protein of human regulator of G-protein signaling 3 (RGS3), transcript variant 2, 20 µg 20 ug
$737.00
TP760493 Purified recombinant protein of Human regulator of G-protein signaling 3 (RGS3), transcript variant 5, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug 50 ug
$270.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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