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M-CSF (CSF1) (NM_172211) Human Over-expression Lysate

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SKU
LY406741
Transient overexpression lysate of colony stimulating factor 1 (macrophage) (CSF1), transcript variant 3 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC217172
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_172211, NP_757350
Synonyms CSF-1; MCSF
Predicted MW 29 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_757350
Locus ID 1435
Cytogenetics 1p13.3
Protein Families WB
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage
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PH305855 CSF1 MS Standard C13 and N15-labeled recombinant protein (NP_757351) 10 ug
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PH317172 CSF1 MS Standard C13 and N15-labeled recombinant protein (NP_757350) 10 ug
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LC403534 Lyophilized CSF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC406741 Lyophilized CSF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC406742 Lyophilized CSF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC424534 Lyophilized CSF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC430359 Lyophilized CSF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY403534 Transient overexpression lysate of colony stimulating factor 1 (macrophage) (CSF1), transcript variant 2 (as WB positive control) 100 ug
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LY406742 Transient overexpression lysate of colony stimulating factor 1 (macrophage) (CSF1), transcript variant 4 (as WB positive control) 100 ug
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LY424534 Transient overexpression lysate of colony stimulating factor 1 (macrophage) (CSF1), transcript variant 1 (as WB positive control) 100 ug
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LY430359 Transient overexpression lysate of colony stimulating factor 1 (macrophage) (CSF1), transcript variant 4 (as WB positive control) 100 ug
$436.00
TP305855 Recombinant protein of human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 4, 20 µg 20 ug
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TP313103 Recombinant protein of human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 2, 20 µg 20 ug
$737.00
TP317104 Recombinant protein of human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 1, 20 µg 20 ug
$737.00
TP317172 Recombinant protein of human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 3, 20 µg 20 ug
$737.00
TP720352 Recombinant protein of human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 1 10 ug
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TP723739 Purified recombinant protein of Human colony stimulating factor 1 (macrophage) (CSF1), transcript variant 4 10 ug
$290.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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