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PD1 (PDCD1) (NM_005018) Human Over-expression Lysate

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SKU
LC401555
Lyophilized PDCD1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
In Stock*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210364
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005018, NP_005009
Synonyms CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2
Predicted MW 31.6 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005009
Locus ID 5133
Cytogenetics 2q37.3
Protein Families WB
Protein Pathways Cell adhesion molecules (CAMs), T cell receptor signaling pathway
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file-alt Citations

Direct therapeutic targeting of immune checkpoint PD-1 in pancreatic cancer
Gao, Lin, Moffitt et al
Br. J. Cancer (2018) 120 (1), 88-96 DOI: 10.1038/s41416-018-0298-0
AI Summary: OriGene’s PD1 (PDCD1) Human Overexpression Lysate and empty vector cell lysate were used as positive and negative controls, respectively, to validate PD-1 expression in pancreatic cancer cells and organoids in this study.
Snippet: Aldrich). PD1 (PDCD1) (NM_005018) Human Overexpression Lysate (Origene) and empty vector cell lysate (Origene) were used as PD-1 positive and negative controls, respectively. Cell line and organoid culture. Human pancreatic
The prognostic role of immune checkpoint markers programmed cell death protein 1 (PD-1) and programmed death ligand 1 (PD-L1) in a large, multicenter prostate cancer cohort
Ness, Andersen, Khanehkenari et al
Oncotarget (2017) 8 (16), 26789-26801 DOI: 10.18632/oncotarget.15817
Cited by 82
AI Summary: OriGene products, specifically the anti-PD-1 (Cat# TA807127) and anti-PD-L1 (Cat# TA592207) antibodies, were used for immunohistochemical detection of PD-1 and PD-L1 expression in prostate cancer tissue samples, enabling the study’s analysis of their prognostic significance.
FigureFigureFigure
Assessing PDL-1 and PD-1 in Non-Small Cell Lung Cancer: A Novel Immunoscore Approach
Paulsen, Kilvaer, Khanehkenari et al
Clin Lung Cancer (2017) 18 (2), 220-233.e8 DOI: 10.1016/j.cllc.2016.09.009
Cited by 88
AI Summary: In this study, OriGene’s anti-PD-L1 antibody (TA307034) and anti-PD-1 antibody (TA811276) were used for immunohistochemical evaluation of PD-L1 and PD-1 expression in tumor samples, enabling the assessment of their prognostic value in non-small cell lung cancer.
The prognostic role of immune checkpoint markers programmed cell death protein 1 (PD-1) and programmed death ligand 1 (PD-L1) in a large, multicenter prostate cancer cohort
Ness, Andersen, Khanehkenari et al
oncotarget (2016)
Snippet: Overexpressed human HEK293T cell lysates were utilized from OriGene for PD-L1 (#LY415473), PD-1 (#LY401555) and HEK293 as empty vector (#LY500001/ negative control). Cells were incubated with 2xSDS sample buffer ... Cite SaveSaving
Assessing PD-L1 and PD-1 in Non-Small Cell Lung Cancer (NSCLC): a Novel Immunoscore Approach
Paulsen, Kilvaer, Khanehkenari et al
Clinical Lung Cancer (2016)
AI Summary: OriGene products, including PD-L1 lysate (#LY415473), PD-1 lysate (#LY401555), HEK293 lysate (#LY500001/negative control), and 2xSDS Sample Buffer, were used as controls and for sample preparation in the assessment of PD-L1 and PD-1 expression in NSCLC.
Snippet: Transient overexpressed human HEK293T cell lysates were utilized from OriGenefor PD-L1(#LY415473), PD-1(#LY401555) and HEK293(#LY500001/negative control);they wereincubated with 2xSDS Sample Buffer (OriGene) for 10 minutes at 100C. Equal amounts ... Cite SaveSaving
Showing 1-5 of 5 papers.
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