PD1 (PDCD1) (NM_005018) Human Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PD1 |
| Synonyms | CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_005018 |
| ORF Size | 864 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_005018.3 |
| RefSeq Size | 2115 bp |
| RefSeq ORF | 867 bp |
| Locus ID | 5133 |
| UniProt ID | Q15116, A0A0M3M0G7 |
| Cytogenetics | 2q37.3 |
| Domains | ig, IG, IGv |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), T cell receptor signaling pathway |
| MW | 31.6 kDa |
| Summary | Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. provided by RefSeq, Aug 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_005018" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210364L1 | Lenti ORF clone of Human programmed cell death 1 (PDCD1), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC210364L2 | Lenti ORF clone of Human programmed cell death 1 (PDCD1), mGFP tagged | 10 ug |
$750.00
|
|
| RC210364L3 | Lenti ORF clone of Human programmed cell death 1 (PDCD1), Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC210364L4 | Lenti ORF clone of Human programmed cell death 1 (PDCD1), mGFP tagged | 10 ug |
$750.00
|
|
| RG210364 | PD-1 / PDCD1 (tGFP-tagged) - Human programmed cell death 1 (PD-1 / PDCD1) | 10 ug |
$489.00
$650.00
|
|
| SC117011 | PD-1 / PDCD1 (untagged)-Human programmed cell death 1 (PD-1 / PDCD1) | 10 ug |
$450.00
|
|
Citations
PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade
Trefny, Kaiser, Stanczak et al
Cancer Immunol Immunother (2020) 69 (8), 1505-1517 DOI: 10.1007/s00262-020-02558-z
Trefny, Kaiser, Stanczak et al
Cancer Immunol Immunother (2020) 69 (8), 1505-1517 DOI: 10.1007/s00262-020-02558-z
Cited by 89
AI Summary: In this study, OriGene products—specifically the anti-PD-1 antibody (Cat# TA347127) and PD-L1 expression plasmid (Cat# RC217445)—were used to detect PD-1 on NK cells and to induce PD-L1 expression in tumor cells, enabling the researchers to investigate how PD-1/PD-L1 interactions affect NK cell function in non-small cell lung cancer.
PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade
Trefny, Kaiser, Stanczak et al
Cancer Immunol Immunother (2020)
Trefny, Kaiser, Stanczak et al
Cancer Immunol Immunother (2020)
Cited by 89
AI Summary: OriGene products pCMV6 human cDNA clone (RC210364) and pCMV-XL4 human cDNA clone (SC115168) were used as sources of PD-1 and PD-L1 open reading frames for cloning expression vectors in this study.
Snippet: Cloning of PD‑1 and PD‑L1 expression vectors The human PD-1/PD-L1 open reading frames (ORFs) were amplified by PCR from a pCMV6 human cDNA clone (RC210364, Origene) and a pCMV-XL4 human cDNA clone (SC115168, Origene), respectively;- Cloning of PD‑1 and PD‑L1 expression vectors The human PD-1/PD-L1 open reading frames (ORFs) were amplified by PCR from a pCMV6 human cDNA clone (RC210364, Origene) and a pCMV-XL4 human cDNA clone (SC115168, Origene), respectively
Targeting glycosylated PD-1 induces potent anti-tumor immunity
Sun, Li, Chung et al
Cancer Res. (2020)
Sun, Li, Chung et al
Cancer Res. (2020)
AI Summary: OriGene’s PD-1 gene plasmid was used to generate PD-1 expression constructs, enabling the study of PD-1 glycosylation and its role in anti-tumor immunity in this paper.
Snippet: CA, USA). 118 119 Plasmids. The PD-1 gene was purchased from Origene (Rockville, MD, USA), and cloned into 120 pCDH lentiviral expression vectors. pCDH-puro-PD-1-FLAG expression vector was used as a 121 template
A Chimeric Switch-Receptor Targeting PD1 Augments the Efficacy of Second-Generation CAR T Cells in Advanced Solid Tumors
Liu, Ranganathan, Jiang et al
Cancer Research (2016) 76 (6), 1578-1590
Liu, Ranganathan, Jiang et al
Cancer Research (2016) 76 (6), 1578-1590
Showing 1-4 of 4 papers.
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