PD1 (PDCD1) (NM_005018) Human Tagged Lenti ORF Clone

SKU
RC210364L3
Lenti ORF clone of Human programmed cell death 1 (PDCD1), Myc-DDK-tagged
  • LentiORF®
    LentiORF®

    Expression-ready ORF plasmid in lenti backbone

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$750.00
In Stock*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PD1
Synonyms CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chl (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC210364).
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_005018
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005018.1
RefSeq Size 2115 bp
RefSeq ORF 867 bp
Locus ID 5133
UniProt ID Q15116
Cytogenetics 2q37.3
Domains IG, ig, IGv
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs), T cell receptor signaling pathway
MW 31.6 kDa
Summary Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity. [provided by RefSeq, Aug 2020]
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SKU Description Size Price
RC210364 PD-1 / PDCD1 (Myc-DDK-tagged)-Human programmed cell death 1 (PD-1 / PDCD1) 10 ug
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$489.00 MSRP $650.00 MSRP $650.00
SC117011 PD-1 / PDCD1 (untagged)-Human programmed cell death 1 (PD-1 / PDCD1) 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.