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CD70 (NM_001252) Human Over-expression Lysate

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SKU
LC400501
Lyophilized CD70 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200410
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_001252, NP_001243
Synonyms CD27-L; CD27L; CD27LG; LPFS3; TNFSF7; TNLG8A
Predicted MW 21.1 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_001243
Locus ID 970
Cytogenetics 19p13.3
Protein Families WB
Protein Pathways Cytokine-cytokine receptor interaction
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file-alt Citations

Characterization of the human T cell response to in vitro CD27 costimulation with varlilumab
Ramakrishna, Sundarapandiyan, Zhao et al
J Immunother Cancer (2015) 3, 37 DOI: 10.1186/s40425-015-0080-2
Cited by 62
AI Summary: In this study, OriGene's Human CD27 (TNFRSF7) cDNA clone (Cat# SC119755) was used to characterize the molecular and functional effects of varlilumab-mediated CD27 costimulation in human T cells.
FigureFigureFigure
Showing 1-1 of 1 papers.
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