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p53 (TP53) Human Gene Knockout Kit (CRISPR)

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SKU
KN400003
TP53 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
  $1,657.00
3 Weeks*

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Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 linear donor
Vector EF1a-GFP-P2A-Puro
Target Symbol p53
Locus ID 7157
Components

KN400003G1, p53 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TCGACGCTAGGATCTGACTG

KN400003G2, p53 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: CGTCGAGCCCCCTCTGAGTC

KN400003D, Linear donor DNA containing LoxP-EF1A-tGFP-P2A-Puro-LoxP:

The sequence below is cassette sequence only. The linear donor DNA also contains proprietary target sequence.

LoxP-EF1A-tGFP-P2A-Puro-LoxP (2739 bp)

ATAACTTCGT ATAATGTATG CTATACGAAG TTATCGTGAG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GTAAGTGCCG TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG CCTTGAATTA CTTCCACCTG GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA AGTGGGTGGG AGAGTTCGAG GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT GAGGCCTGGC CTGGGCGCTG GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT CTCGCTGCTT TCGATAAGTC TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT TTTTTCTGGC AAGATAGTCT TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT TTTGGGGCCG CGGGCGGCGA CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG GGCCTGCGAG CGCGGCCACC GAGAATCGGA CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT CTGGTGCCTG GCCTCGCGCC GCCGTGTATC GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG TCGGCACCAG TTGCGTGAGC GGAAAGATGG CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA AAATGGAGGA CGCGGCGCTC GGGAGAGCGG GCGGGTGAGT CACCCACACA AAGGAAAAGG GCCTTTCCGT CCTCAGCCGT CGCTTCATGT GACTCCACGG AGTACCGGGC GCCGTCCAGG CACCTCGATT AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT TATGCGATGG AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC TTGATGTAAT TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAT GGAGAGCGAC GAGAGCGGCC TGCCCGCCAT GGAGATCGAG TGCCGCATCA CCGGCACCCT GAACGGCGTG GAGTTCGAGC TGGTGGGCGG CGGAGAGGGC ACCCCCGAGC AGGGCCGCAT GACCAACAAG ATGAAGAGCA CCAAAGGCGC CCTGACCTTC AGCCCCTACC TGCTGAGCCA CGTGATGGGC TACGGCTTCT ACCACTTCGG CACCTACCCC AGCGGCTACG AGAACCCCTT CCTGCACGCC ATCAACAACG GCGGCTACAC CAACACCCGC ATCGAGAAGT ACGAGGACGG CGGCGTGCTG CACGTGAGCT TCAGCTACCG CTACGAGGCC GGCCGCGTGA TCGGCGACTT CAAGGTGATG GGCACCGGCT TCCCCGAGGA CAGCGTGATC TTCACCGACA AGATCATCCG CAGCAACGCC ACCGTGGAGC ACCTGCACCC CATGGGCGAT AACGATCTGG ATGGCAGCTT CACCCGCACC TTCAGCCTGC GCGACGGCGG CTACTACAGC TCCGTGGTGG ACAGCCACAT GCACTTCAAG AGCGCCATCC ACCCCAGCAT CCTGCAGAAC GGGGGCCCCA TGTTCGCCTT CCGCCGCGTG GAGGAGGATC ACAGCAACAC CGAGCTGGGC ATCGTGGAGT ACCAGCACGC CTTCAAGACC CCGGATGCAG ATGCCGGTGA AGAAAGAGGA AGCGGAGCTA CTAACTTCAG CCTGCTGAAG CAGGCTGGAG ACGTGGAGGA GAACCCTGGA CCTATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAATAAC TTCGTATAAT GTATGCTATA CGAAGTTAT

More linear donor DNA can be ordered separately

Cat# KN400003D
Description 10 ug linear donor DNA for KN400003 kit
Price $515/€515
If you want to order 20ug, please order quantity of 2 for KN400003D
If you want to order 50ug, please order quantity of 5 for KN400003D

Other selection marker is available for donor DNA.
Please contact techsupport@origene.com for a custom quote.

different donor dna img
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_000546, NM_001126112, NM_001126113, NM_001126114, NM_001126115, NM_001126116, NM_001126117, NM_001126118, NM_001276695, NM_001276696, NM_001276697, NM_001276698, NM_001276699, NM_001276760, NM_001276761
UniProt ID P04637
Synonyms BCC7; LFS1; P53; TRP53
Summary This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). provided by RefSeq, Dec 2016
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Documents
Resources
Other Versions
SKU Description Size Price
GA104942 TP53 CRISPRa kit - CRISPR gene activation of human tumor protein p53 1 kit
$1,657.00
KN200003 TP53 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN200003BN TP53 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN200003LP TP53 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN200003RB TP53 - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00

file-alt Citations

Hotspot mutant p53-R273H enhances mitochondrial biogenesis and cell migration in primary colorectal cancer in response to oxaliplatin
Martinez-Bernabe, Morla-Barcelo, Fiore et al
Biochim Biophys Acta Mol Cell Res (2025)
Cited by 3
AI Summary: OriGene’s CRISPR TP53 human knockout kit (#KN400003) was used to generate TP53 knockout SW480 colorectal cancer cells, enabling the study of how p53-R273H mutation affects mitochondrial biogenesis and cell migration in response to oxaliplatin.
Snippet: Knockout of TP53 was performed using the CRISPR TP53 human knockout kit (#KN400003;OriGene, Rockville, USA) in SW480 cell line
iNOS/NO is required for IRF1 activation in response to liver ischemia-reperfusion in mice
Du, Luo, Yang et al
Mol. Med. (2020) 26 (1), 56 DOI: 10.1186/s10020-020-00182-2
Cited by 17
AI Summary: OriGene products, specifically the human TP53 and IRF1 expression plasmids (pCMV6-xl5-TP53 and pCMV6-xl5-hIRF1), were used in this study to investigate the roles of these genes in iNOS/NO-mediated IRF1 activation and PUMA expression during liver ischemia-reperfusion in mice.
Snippet: Human TP53 and IRF1 expression plasmids, pCMV6-xl5-TP53 and pCMV6-xl5-hIRF1 were purchased from Origene, Rockville, MD
FigureFigureFigure
Inhibition of the formation of autophagosome but not autolysosome augments ABT-751-induced apoptosis in TP53-deficient Hep-3B cells
Wei, Wu, Pan et al
J. Cell. Physiol. (2018)
AI Summary: OriGene's pCMV6-TP53 (RC2000003) plasmid was used to exogenously express the TP53 gene in Hep-3B cells, enabling the study of TP53’s role in ABT-751-induced apoptosis and autophagy.
Snippet: To exogenously express the TP53 gene, cells (1.5 × 10 5 ) were seeded in six‐well plates overnight and transfected with 1 μg of pCMV6‐Entry (control), pCMV6‐TP53 (RC2000003;OriGene, Rockville, MD) or pHRIG‐AKT1 (#53583;Addgene, Cambridge, MA)
A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells
Wei, Lin, Wu et al
Toxicol. Appl. Pharmacol. (2016)
Cited by 22
AI Summary: In this study, OriGene products contributed by providing the DDK antibody (TA50011) for immunoblotting and the pCMV6-TP53 plasmid (RC200003) for TP53 gene overexpression experiments, which were essential for investigating the molecular mechanisms of ABT-751-induced autophagy and apoptosis in Huh-7 cells.
Snippet: 1 (SQSTM1, also known as p62, 1:500, sc-28,359, Santa Cruz), autophagy related 5 (ATG5, 1:1000, GTX102361, GeneTex), ATG12 (1:1000, GTX124181, GeneTex), beclin 1, autophagy related (BECN1, 1:1000, GTX113039, GeneTex), DDK (1:1000, TA50011, Origene ... … To exogenously express the TP53 gene, cells (1.5 105) were seeded in 6-well plates overnight and transfected with 1 ?g of pCMV6-Entry (control) or pCMV6-TP53 (RC200003, Origene) plasmid by mixing with 3 ?L PolyJet reagent for 16 h, replaced with normal medium and incubated for another 8 h before analysis
Molecular mechanism leading to SAHA-induced autophagy in tumor cells: evidence for a p53-dependent pathway
Fröhlich, Mrakovcic, Smole et al
Cancer Cell Int. (2016) 16 (1), 68 DOI: 10.1186/s12935-016-0343-0
Cited by 35
AI Summary: In this study, the pCMV6-XL5 expression vector containing human full-length TP53 cDNA (TP53-WT, SC119832) from OriGene was used to reintroduce wild-type p53 into ESS-1 cells, enabling the researchers to assess the role of p53 in regulating SAHA-induced autophagy and apoptosis.
Snippet: Protein expression was quantified using Image J software. TP53 gene transfer. The expression vector pCMV6-XL5 containing the human full-length cDNA for TP53 (TP53-WT) (NCBI Acc.-No: NM_000546.2;SC119832) was obtained from OriGene (Rockville, CA, USA ... Cite SaveSaving
FigureFigureFigure
Estudio del efecto de la mutación P53-R273H sobre la función mitocondria en el cáncer de cólon en el contexto de un ambiente inflamatorio
Cara
dspace.uib.es (2024)
Snippet: Las líneas knockout para TP53 (TP53 KO) se obtuvieron por reparación no homóloga mediada por CRISPR/Cas9. Se utilizó CRISPR TP53 human knockout kit (#KN400003, OriGene, Rockville, MD, EEUU) en las líneas celulares SW480 y SW620 antes mencionadas. El kit contiene un vector donante con un cassette funcional de GFP-puromicina y dos vectores Cas9
Showing 1-6 of 6 papers.
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