Mcam Mouse Gene Knockout Kit (CRISPR)

CAT#: KN309859RB

Mcam - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: Mcam
• Locus ID: 84004
• Components:

  KN309859G1, Mcam gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CGCCCGACCTGGTGGAGGCA

  KN309859G2, Mcam gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGTGGCCCCTCACGGGCCTC

  KN309859RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  Homologous arm and RFP-BSD sequences:

  pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

  CTGACGTCAC TCCTGCCCAC TCTCTGGTTT TTGACCTAAC CCTTGGCGGA GACTGACCCC TCTAGACTTG GATGGGGTCT TCAGATTGTA TTGCAGCGTT TCTTGACATG CTACTGTGGG AACATAAATC ATAGCCTCTG GCTAGAGTTG GCTCACTGTG TCTTCCAGAG GATTTGGGGG ACAGAGGGTA GAATAGTGAC GTCTTCGTGG AGGGGGGGCA CACAAAGGGG CCTGTGTGCC CCAGCCTGGC ACATGCCTTA CATTCCCCTA GTCTGAGCTC ACCAGGACTA GGAAGGGGCC CAGGAGCAAA GGGGCCTTCA TCTTGGCCCA GAGCCCATGT TGCTCTTATC TGTCTCTCCA CCAGCTTCTT CTAAGATATA CCCAGTCACT CTGTCTGTGT TTCACTCCGT ATTACACTGG GAGAGGTCTG ACCCAGGTTG GGGGTGTGGG CTTGGTGAAG TTGAGGGTAA GGAGAGGGTA AGGCCCTCCC CAGACCCTTC AAGTGGGACA GCTGCAGTGA ACCAGGAGAG GGGAGGGGAC CGGACAGAAC ACCTGGAGCT GACAAAGCTG CCTGGGCATG CCCCTCTCTT TCCCCAGGTG CAACTTCAGC CAAGTGGACT GGTTTTTGGT GAGCGCCTGG ACTTTGGAGG GCAGTAGTTG TGTGGGAAGC AACTGTCTCT CTGGCAGGTA GCTCATCTCT CAGCTCCTTT CCCTCAGATT CACAAGGAGA GGCAGATACT GATTTTCCGT GTGCACCAAG GCAAGGGCCA GCGGGAACCT GGTGAATATG AGCACCGCCT TAGCCTCCAA GACTCGGTGG CTACTCTGGC CCTGAGTCAC GTCACTCCCC ATGATGAGCG AATGTTCCTG TGTAAGAGCA AGCGACCACG GCTCCAGGAT CACTACGTTG AGCTTCAGGT CTTCAGTGAG TGCCCCCCTC GTTTCTGCCT TCAGGGTCGT GTGGACCATG TAGCAAAGAT GGGTCTCCAT GTCCCTGTTC ATTCCCCTTC CACTGTAGAA GCCCCAGAGG AACCAACTAT TCAAGCCAAT GTCGTGGGCA TCCATGTGGA CAGGCAAGAG CTCAGGGAGG TGAGAGGCAC TCGGTCAAAT GATGGTGGAT AGAGGCTAAG CAGGGACGGG GGTGGGGGGG GGTGGTATGT GGCAGACGCA AGGAGACTCT CGTCCTGTCC TCCTTAGGTT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_023061, NM_001359530
• UniProt ID: Q8R2Y2
• Synonyms: 1-gicerin; AV025631; CD146; CD149; Muc18; s-endo; s-gicerin
• Summary: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).[UniProtKB/Swiss-Prot Function]