Mcam Mouse Gene Knockout Kit (CRISPR)

CAT#: KN309859

Mcam - mouse gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: Mcam
• Locus ID: 84004
• Components:

  KN309859G1, Mcam gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CAACATAAAGAACTCACCCG

  KN309859G2, Mcam gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TGATCTCCGGTACACTCAGG

  KN309859D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AAGGCACCGT TTCCAACTCT CCCGCCCCCT TGCAGGCACC CCTAGGTCTG TGGGACCTGG CACAGTTCTT CCTCCAAGGC TGCCAGGCCA GCGAAGACTC TGGAACAGTG CCAAGCCGGC AGCCGCCGCA GTGAAGCGGT CATTCCAGAA GGCGACCAGG GCCAGCTCCA TGCCTCCACA GCAGGGTAGA GGAACCTGAC TGAGAGTCCT CTATCAGGGA TACCTCTCTG TTGCTCCTCT GCTAGAGGGT CCCCGCTAGT AGTGGACAAA CCTGAGCGAG CGCTGCTACT GCGTCCAAGA GTCTACAGCG ACCGGGCCAG CTTCTGCCGC CTCGGCCAGT CATTCGAGCC CCAGGACACC TGTCATTGCT CCTTCAACCT TTCCAAGCCA GAAAACGGGA GTTAGCCTCT AGGCCTGGAT CGGGGAGCAG CAGCTCGCAG CCAGCGCGCC GGGGGCCCGC CCCCTAGGGC GCGGGGAGGG GGCGGGGGGC TACGCGGGGC GGGGCCGGGC GGCGCCCGGG GTCCCCTCCC CCGCCCCCCT GACGTCAGCC CCCGACAGCG GCGAGTTCCT CACTTGGCTC GCGCCCTCCG TCGGGGAAGC TCGGGGCTCT GCACCCCAGT GGGCGCTCCT TCTTGCCAGA GTCCCCGATT TCCAGGCGCC CCGCGTGCGT GCAACTTCTT TGCGGGTGTG CAGCCTAGGT TTTAGAGTAT TCTGGGAACG GACTAGTCGG GTTGCTGCAG CAGCGAGCGG GAAGGGGTGG GGCCGAGCTT CTGCAGGACG CTGGGCCCGG CCTCCCGCAG GTGTTCGCTC TGGGTGCTGG ATGCTGGGTG CTGGAGTCCC GGCAGAGAGG CGCAGCCGGG GCGCATCCCC AACCCGTGCA TCCCGCGCCC GCCTGTTGCT GGAAAAGGCA GTTTCCCTAG CTGGAGGGGG CCGCCGACCC CTCCCCCTCC GCCTGGGCCT CACGCCAGTT TGGAGGTGAG CGAGGGAGGA GGCCGCTCCT GCTGGGGCTG GGCGTTGGCA AGGGCAGCGG GTGGTGTGGG TCTCCTTGCG CCTCCGGGTT TGACGGGATT ACAGGGGAAA GACAACAGAT GGAGAAGTTC TACAACAGTA ACAGCTTCTG TGGTCCACTA GAGTCAGGTG GAGAGAGCCT GGCACCTCGG AGCTTTCCTT TAAGCCATCG AGTAGACATG CTTTCCTGGG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_023061, NM_001359530
• UniProt ID: Q8R2Y2
• Synonyms: 1-gicerin; AV025631; CD146; CD149; Muc18; s-endo; s-gicerin
• Summary: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).[UniProtKB/Swiss-Prot Function]