mtTFA (TFAM) Human Gene Knockout Kit (CRISPR)

SKU KN215488RB

TFAM - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Vector: RFP-BSD
• Target Symbol: mtTFA
• Locus ID: 7019
• Components:

  KN215488G1, mtTFA gRNA vector 1 in pCas-Guide CRISPR vector

  KN215488G2, mtTFA gRNA vector 2 in pCas-Guide CRISPR vector

  KN215488RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001270782, NM_003201, NM_012251, NR_073073
• UniProt ID: Q00059
• Synonyms: MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3
• Summary: This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
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