PD-L1 (CD274) Human Gene Knockout Kit (CRISPR)

SKU KN213071LP

CD274 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Vector: Luciferase-Puro
• Target Symbol: PD-L1
• Locus ID: 29126
• Components:

  KN213071G1, PD-L1 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TCTTTATATTCATGACCTAC

  KN213071G2, PD-L1 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: ACCGTTCAGCAAATGCCAGT

  KN213071LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  AATCTGTTTA TTGAGAGAAT AGATTTATAA TTTAGAATAA GTGAGAGGGG CAGAAGAGGA GATTTTGAAG GATGGCACCT GAAGGAGGAC TAGCATGGCT GAGACAGTGA AGTGGAAGCC TTGAATAGCT AAAGGGTAAG ATGAAAGTAT TTAGCTGTAG GGGGAAAAAG CATTGACAGG TTGGAAAAGT AAAAGTCAGA TTCTCCTTGC TCTGAAATTT TGTACAGGGC AGGTTCTACT AGGTATGTTA CAATGCAGAA AAAACATGAA ATAATTGAGA GGAATTTGGT GCAATATTAT CTTCTTGGCT TCTTTTGAGT GGGCAGATTT TTTTCACGGC CTGTAACTAT AATAAATTTG AAACTTCTCA TCTTTTAGTA ACTTTTTTCA CTTAAGTTTA TGTGGCTGTG GGCAATGGAA TGAAGATATT GAACTTCCAA TTCCCTGTTG GGTTTCCACA ATTACAAGTC AATCATGACT GGTTATTAGA AGACTATTTC AGTTAGAACC ACCAAGTCCC ATATTGTCAT ATTGTATGTT TAATTATTAA GTGAAGCAGT CTTCTTTTCG TGTTTTCCAT AATTAGGGCA TTCCAGAAAG TGAGTTTAAA ACTAAAATGA TCATTTAAAA TGCATGCAAT TTTCTTATAG AGAGAACATT CTATTCTTTC TTCTACTTTA CACAATGGCA AAGTCTTCTT TCTACTTTAC GCAATGATAA AGTTACCTGT GTCATTTTGT AAAAATATAG AGAATATAGA CAAATTGAAA GACACAAAAT AATCTATTAC CCATTTCCCA GGGTTAACTA CTGAAAATAT CTGGGGAAAT GGCCTGTATG TATACATTTA TTTGTTTGCT TTCAACAAGG CCAAGATCCT TTGATCTTTC AGTCTTGGTT GCTCTGTGAC ATGCCTTTCC TGATGAGGAT ACTTTAAGGA AGAATTGTAA GATACATGGA AAATGTCAGG CTAACACAGT ACTGGCATCA CCCTGTGCTC TTTCCTGAAC TCCATACCAA TGTACTTCTT GCCAGAAAAC TGATCAAAAG TTTAGGGAAG TAAAAAGAGA TGACTGTTAG AATCTACCAT TCCCTCTATG TAGGAAGCAA ATAGGTGTCC TGTCAAAGGA CATTCTGGGG ATGTCTACAT GAAACCAAGT CTCCCTGGTT GTAAGGACTC CATCTCCATA TAATATTTAT

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001267706, NM_001314029, NM_014143, NR_052005
• UniProt ID: Q9NZQ7
• Synonyms: B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1
• Summary: This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
• Shipping: Ambient