BCDIN3D Human Gene Knockout Kit (CRISPR)

CAT#: KN208818

BCDIN3D - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
• Donor DNA: GFP-puro
• Symbol: BCDIN3D
• Locus ID: 144233
• Components:

  KN208818G1, BCDIN3D gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGAAGAGGAATCGCGAGTTC

  KN208818G2, BCDIN3D gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTTCCGAACGGGGCGGCGCC

  KN208818D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

  Homologous arm and GFP-puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

  AAGTGCTGGC TCTTAAGGCA CGGTCCTTGC CTCTCAAGGA GCTCATGGTC TACAGTTGCC CCTGGGGGAC AGGGCTGAGT CGGCTGGCTT CGCAAGTGAC CTGCACCTCC TGGCTACCCT TTCCTGAACC GGGTGGAAGT TTTTGAGTCA CAGATTCAAT GTATGACCCC AGACAAGTAA GTTCTCTCGG TCTCACTTTT TGCATTTATA AAGAAAGGAA ATTGGACTAA AATTGATTTC CAAGATCCCA ACAAACTAGT ATACTTGTTC TGGGTCAAGT AGCCCAAGTT TCAAACTATG TCAGCTTGGC CAAGACACTT AGCCCTCAGA GTTTGTTTCA TCCCTGTAAA ACGTGGATGA TATTGCTAAT CCCCTCCCAG TGTTGTGATG AGGGTTCAAT AATACAAGGC AAGCCCCAGG CGCTCGCCGG CTCCAGGTAT ACAGCGAGAG GAAATCAGGA CAGCTTTTCT GATTCACCGC AGAATTCTAG CCACCCGCCC TAGCCGTTCG ACGCACGCGC GCTGGCACCG CCACCGCCCA CGCCGACAGC AGGTTCCCGG CTTCCGGTTC CGCCACGGCC CAGAGGCTGT GTTGAGGCTA CTCCGCCTCC TGCCCCCGGA GCTGCTTCGA CAGCTCTTTC CTGAGAGTCC CGAGAACGGG CCGATTCTGG GGCTCGACGT GGGGTGTAAC TCCGGGGTGA GTGACAGGGG AGGGATTGGA GCATCCGGAG CCAAGTGGTT GGCTGCCTAC ACATCCACTG TGGTTCTGGC CTGGGCTAAC ACCCATCGAA ACTTTCCCAA AACGAGTATA AAGCGTGGCT TTGACTGAGC CAGTGCCGCT GCTGCCTGCC CGCCAGGGTT CCACCATCTG TCTTAGGAAT CTCTCTTGGA GAGGAGTCCT TGTCGACTAC GTTCCCACTT TTCTTTCAGG GCCAGACCCC AGGTGGGAAC GGTCCTTGTA TCTAGGGTGG CCTCTGTCCA GCGTGCCTGG CGCTTTGGCT GGGGAGAGGG GGCTTCCAGA GACACACGTT TGGGAACATG GGGTTAGGTT TGGTTTACTG GGAGAGAAGA GCCAAGGGTG ACAGAATCAT GCCTGATATT GAGACTCATG TGTGCTATAC AGTGCAGGAC AGACCCTGGG ACCTGCATGT TTATTTCAAA AAAAGTGAGT AGGCCGGGCG CGCTGGCTCA CGCCTGTAAT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_181708
• UniProt ID: Q7Z5W3
• Synonyms: BCDIN3 domain containing
• Summary: This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family, and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. [provided by RefSeq, Apr 2017]