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BCDIN3D (NM_181708) Human Tagged ORF Clone

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SKU
RC208818
BCDIN3D (Myc-DDK-tagged)-Human BCDIN3 domain containing (BCDIN3D)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "BCDIN3D or NM_181708"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BCDIN3D
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC208818 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTGCCCACGGAACTGGATGGAGGGAGTGTTAAGGAGACCGCAGCGGAAGAGGAATCGCGAGTTC
TGGCACCTGGCGCCGCCCCGTTCGGAAATTTTCCTCATTATTCTCGCTTCCACCCTCCGGAGCAACGGCT
CCGCCTCCTGCCCCCGGAGCTGCTTCGACAGCTCTTTCCTGAGAGTCCCGAGAACGGGCCGATTCTGGGG
CTCGACGTGGGGTGTAACTCCGGGGATCTGAGTGTGGCTCTATACAAACACTTCCTCTCCCTACCTGACG
GGGAAACCTGCTCAGATGCCTCAAGAGAATTCCGTCTCCTCTGCTGCGACATAGATCCAGTCCTGGTGAA
GCGAGCCGAAAAAGAATGTCCTTTTCCTGATGCCTTGACTTTTATCACCCTGGACTTCATGAATCAAAGG
ACCCGGAAGGTTCTCTTGAGCTCTTTCTTAAGCCAATTTGGACGTTCAGTTTTTGACATTGGCTTCTGCA
TGTCAATAACCATGTGGATTCATCTGAATCATGGAGACCATGGCCTATGGGAGTTCCTGGCCCATCTTTC
CTCCCTCTGCCACTACCTCCTTGTGGAGCCCCAACCCTGGAAGTGTTACCGGGCAGCTGCAAGGCGTCTC
CGAAAGCTGGGACTCCATGATTTTGACCACTTCCACTCCCTTGCCATCCGAGGTGACATGCCCAATCAGA
TTGTGCAGATCTTGACCCAGGATCATGGCATGGAATTAATATGTTGCTTTGGCAACACCAGTTGGGACAG
AAGCCTTCTGCTCTTCAGGGCAAAACAAACCATAGAGACTCATCCAATCCCTGAATCACTGATAGAAAAA
GGGAAAGAAAAGAACAGATTAAGTTTCCAGAAGCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC208818 protein sequence
Red=Cloning site Green=Tags(s)

MAVPTELDGGSVKETAAEEESRVLAPGAAPFGNFPHYSRFHPPEQRLRLLPPELLRQLFPESPENGPILG
LDVGCNSGDLSVALYKHFLSLPDGETCSDASREFRLLCCDIDPVLVKRAEKECPFPDALTFITLDFMNQR
TRKVLLSSFLSQFGRSVFDIGFCMSITMWIHLNHGDHGLWEFLAHLSSLCHYLLVEPQPWKCYRAAARRL
RKLGLHDFDHFHSLAIRGDMPNQIVQILTQDHGMELICCFGNTSWDRSLLLFRAKQTIETHPIPESLIEK
GKEKNRLSFQKQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181708
ORF Size 876 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181708.3
RefSeq Size 3266 bp
RefSeq ORF 879 bp
Locus ID 144233
UniProt ID Q7Z5W3
Cytogenetics 12q13.12
MW 33.2 kDa
Summary This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family, and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. provided by RefSeq, Apr 2017
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"NM_181708" in other vectors (6)
SKU Description Size Price
RC208818L1 Lenti ORF clone of Human BCDIN3 domain containing (BCDIN3D), Myc-DDK-tagged 10 ug
$600.00
RC208818L2 Lenti ORF clone of Human BCDIN3 domain containing (BCDIN3D), mGFP tagged 10 ug
$600.00
RC208818L3 Lenti ORF clone of Human BCDIN3 domain containing (BCDIN3D), Myc-DDK-tagged 10 ug
$600.00
RC208818L4 Lenti ORF clone of Human BCDIN3 domain containing (BCDIN3D), mGFP tagged 10 ug
$600.00
RG208818 BCDIN3D (tGFP-tagged) - Human BCDIN3 domain containing (BCDIN3D) 10 ug
$489.00 $500.00
SC126373 BCDIN3D (untagged)-Human BCDIN3 domain containing (BCDIN3D) 10 ug
$300.00

Plasmid Map for RC208818

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC208818
Circular map for RC208818

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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