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BCDIN3D Human Gene Knockout Kit (CRISPR)

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SKU
KN208818
BCDIN3D - human gene knockout kit via CRISPR, HDR mediated
  $1,657.00
4 Weeks*

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Other products for "BCDIN3D or NM_181708.3"

Specifications
Specifications
Product Data
Format 2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Vector GFP-puro
Target Symbol BCDIN3D
Locus ID 144233
Components

KN208818G1, BCDIN3D gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: GGAAGAGGAATCGCGAGTTC

KN208818G2, BCDIN3D gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: TTTCCGAACGGGGCGGCGCC

KN208818D, donor DNA containing left and right homologous arms and GFP-puro functional cassette.

Homologous arm and GFP-puro sequences:

pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet

AAGTGCTGGC TCTTAAGGCA CGGTCCTTGC CTCTCAAGGA GCTCATGGTC TACAGTTGCC CCTGGGGGAC AGGGCTGAGT CGGCTGGCTT CGCAAGTGAC CTGCACCTCC TGGCTACCCT TTCCTGAACC GGGTGGAAGT TTTTGAGTCA CAGATTCAAT GTATGACCCC AGACAAGTAA GTTCTCTCGG TCTCACTTTT TGCATTTATA AAGAAAGGAA ATTGGACTAA AATTGATTTC CAAGATCCCA ACAAACTAGT ATACTTGTTC TGGGTCAAGT AGCCCAAGTT TCAAACTATG TCAGCTTGGC CAAGACACTT AGCCCTCAGA GTTTGTTTCA TCCCTGTAAA ACGTGGATGA TATTGCTAAT CCCCTCCCAG TGTTGTGATG AGGGTTCAAT AATACAAGGC AAGCCCCAGG CGCTCGCCGG CTCCAGGTAT ACAGCGAGAG GAAATCAGGA CAGCTTTTCT GATTCACCGC AGAATTCTAG CCACCCGCCC TAGCCGTTCG ACGCACGCGC GCTGGCACCG CCACCGCCCA CGCCGACAGC AGGTTCCCGG CTTCCGGTTC CGCCACGGCC CAGAGGCTGT GTTGAGGCTA CTCCGCCTCC TGCCCCCGGA GCTGCTTCGA CAGCTCTTTC CTGAGAGTCC CGAGAACGGG CCGATTCTGG GGCTCGACGT GGGGTGTAAC TCCGGGGTGA GTGACAGGGG AGGGATTGGA GCATCCGGAG CCAAGTGGTT GGCTGCCTAC ACATCCACTG TGGTTCTGGC CTGGGCTAAC ACCCATCGAA ACTTTCCCAA AACGAGTATA AAGCGTGGCT TTGACTGAGC CAGTGCCGCT GCTGCCTGCC CGCCAGGGTT CCACCATCTG TCTTAGGAAT CTCTCTTGGA GAGGAGTCCT TGTCGACTAC GTTCCCACTT TTCTTTCAGG GCCAGACCCC AGGTGGGAAC GGTCCTTGTA TCTAGGGTGG CCTCTGTCCA GCGTGCCTGG CGCTTTGGCT GGGGAGAGGG GGCTTCCAGA GACACACGTT TGGGAACATG GGGTTAGGTT TGGTTTACTG GGAGAGAAGA GCCAAGGGTG ACAGAATCAT GCCTGATATT GAGACTCATG TGTGCTATAC AGTGCAGGAC AGACCCTGGG ACCTGCATGT TTATTTCAAA AAAAGTGAGT AGGCCGGGCG CGCTGGCTCA CGCCTGTAAT

GE100003, scramble sequence in pCas-Guide vector
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq NM_181708
UniProt ID Q7Z5W3
Synonyms BCDIN3 domain containing
Summary This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family, and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. provided by RefSeq, Apr 2017
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Documents
Resources
Other Versions
SKU Description Size Price
GA115487 BCDIN3D CRISPRa kit - CRISPR gene activation of human BCDIN3 domain containing RNA methyltransferase 1 kit
$1,657.00
KN208818BN BCDIN3D - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN208818LP BCDIN3D - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN208818RB BCDIN3D - human gene knockout kit via CRISPR, HDR mediated 1 kit
$1,657.00
KN408818 BCDIN3D - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated. 1 kit
$1,657.00

file-alt Citations

ChemRAP uncovers specific mRNA translation regulation via RNA 5' phospho-methylation
Ipas, Gouws, Abell et al
EMBO Rep (2024)
AI Summary: OriGene’s BCDIN3D Human Gene Knockout Kit (CRISPR) (#KN208818) was used to generate BCDIN3D-deficient HeLa cell lines, enabling the study of how BCDIN3D regulates mRNA translation via RNA 5′ phospho-methylation in this research.
Snippet: 5′-end modifications play key roles in determining RNA fates. Phospho-methylation is a noncanonical cap occurring on either 5′-PPP or 5′-P ends. We used;BCDIN3D was knocked out in HeLa and HeLa-S3-FlpIn cells with the BCDIN3D Human Gene Knockout Kit (CRISPR) from Origene (#KN208818) as recommended. The puromycin concentration used for the candidate clone selection step was at 1 µg/µL
Showing 1-1 of 1 papers.
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