Osteopontin (SPP1) Human Gene Knockout Kit (CRISPR)

SKU KN204803RB

SPP1 - human gene knockout kit via CRISPR, HDR mediated

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Vector: RFP-BSD
• Target Symbol: Osteopontin
• Locus ID: 6696
• Components:

  KN204803G1, Osteopontin gRNA vector 1 in pCas-Guide CRISPR vector

  KN204803G2, Osteopontin gRNA vector 2 in pCas-Guide CRISPR vector

  KN204803RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• OTI Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000582, NM_001040058, NM_001040060, NM_001251829, NM_001251830
• UniProt ID: P10451
• Synonyms: BNSP; BSPI; ETA-1; OPN
• Summary: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Shipping: Ambient