Osteopontin (SPP1) Mouse Monoclonal Antibody [Clone ID: OTI5E4]
$447.00
In Stock*
Specifications
| Product Data | |
| Clone Name | OTI5E4 |
|---|---|
| Application | IHC, WB |
| Recommended Dilution | WB 1:2000, IHC 1:150 |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 17-314 of human SPP1(NP_001035147) produced in E.coli. |
| Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide |
| Concentration | 1 mg/ml |
| Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Predicted Protein Size | 33.7 kDa |
| Gene Name | secreted phosphoprotein 1 |
| Database Link | |
| Background | The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2011 |
| Synonyms | BNSP; BSPI; ETA-1; OPN |
| Reference Data | |
| Protein Categories | Cytokines, Secreated Proteins |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other "OTI5E4" antibodies (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| CF806784 | Carrier-free (BSA/glycerol-free) SPP1 mouse monoclonal antibody, clone OTI5E4 (formerly 5E4) | 100 ug |
$620.00
|
|
| TA806784AM | SPP1 mouse monoclonal antibody, clone OTI5E4 (formerly 5E4), Biotinylated | 100 ul |
$509.00
|
|
| TA806784BM | SPP1 mouse monoclonal antibody, clone OTI5E4 (formerly 5E4), HRP conjugated | 100 ul |
$509.00
|
|
| TA806784S | SPP1 mouse monoclonal antibody, clone OTI5E4 (formerly 5E4) | 30 ul |
$200.00
|
|
Citations
Spatial transcriptomics reveals unique metabolic profile and key oncogenic regulators of cervical squamous cell carcinoma
Zhou, Liu, Yao et al
J Transl Med (2024) 22 (1), 1163 DOI: 10.1186/s12967-024-06011-y
Zhou, Liu, Yao et al
J Transl Med (2024) 22 (1), 1163 DOI: 10.1186/s12967-024-06011-y
Cited by 12
AI Summary: In this study, the OriGene SPP1 antibody (cat #TA806784) was used for immunohistochemical analysis to assess protein expression patterns in cervical squamous cell carcinoma tissues, aiding in the spatial profiling of tumor metabolism and microenvironment.
Snippet: night incubation at 4 °C with the primary antibody. The primary antibodies are as follows: (1) IDO1 (HUABIO;cat #HA721331;1:200), (2) CD274/PD-L1 (Proteintech;cat #66248-1-Ig;1:5000), (3) SPP1 (OriGene;cat #TA806784;1:150). After washing with PBS, the slices were incubated with species-specific HRP labeled secondary antibodies for 50 min at room temperature. The secondary antibodies are g
Injectable angiogenic and osteogenic carrageenan nanocomposite hydrogel for bone tissue engineering
Yegappan, Selvaprithiviraj, Amirthalingam et al
Int. J. Biol. Macromol. (2019) 122, 320-328 DOI: 10.1016/j.ijbiomac.2018.10.182
Yegappan, Selvaprithiviraj, Amirthalingam et al
Int. J. Biol. Macromol. (2019) 122, 320-328 DOI: 10.1016/j.ijbiomac.2018.10.182
AI Summary: OriGene Technologies’ mouse anti-OPN antibody was used in this study to detect osteopontin protein expression during the evaluation of osteogenic differentiation in rat adipose-derived mesenchymal stem cells.
Snippet: Expression of bone specific protein such as RUNX-2, osteopontin (OPN) and collagen (COL) was analysed by immunofluorescence. rADSCs were cultured as in Section 2.12. Cell fixation with 4% PFA followed by permeabilization with 0.5% triton X-100 in PBS and subsequent blocking with 3% BSA in PBS for 30 min. Primary antibodies of RUNX-2 (goat anti-RUNX-2, Pierce Antibodies, USA), OPN (mouse anti-OPN, ...
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
