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env (NC_001802) Virus Tagged ORF Clone

  • TrueORF®
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SKU
VC101721
Myc-DDK-tagged ORF clone of viral ORF for Envelope surface glycoprotein gp160, precursor [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057856
$589.00 $784.00
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Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol env
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC101721 represents NCBI reference of NP_057856 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGTTAAGGAAAAATATCAGCACCTCTGGAGGTGGGGATGGAGATGGGGCACTATGCTTCTCGGGA
TGCTCATGATTTGCAGCGCAACCGAAAAGCTGTGGGTGACGGTATACTACGGCGTTCCAGTCTGGAAAGA
GGCAACTACAACCCTTTTTTGCGCTTCCGACGCCAAGGCATATGACACTGAAGTCCATAACGTGTGGGCA
ACCCACGCCTGTGTCCCAACGGATCCAAATCCCCAGGAAGTTGTATTGGTTAATGTGACTGAGAACTTTA
ACATGTGGAAAAACGACATGGTCGAACAAATGCATGAAGATATTATTTCACTGTGGGATCAATCTCTGAA
ACCTTGCGTCAAGCTGACACCTCTCTGTGTCTCACTTAAATGTACCGATCTGAAGAACGACACTAACACT
AATTCTTCTAGCGGTAGAATGATCATGGAAAAGGGCGAGATCAAGAACTGCTCCTTTAATATTAGTACCA
GTATTCGGGGTAAGGTCCAAAAGGAGTACGCTTTCTTTTACAAGCTCGATATTATTCCCATTGATAACGA
TACCACCAGTTATAAACTGACTTCTTGTAACACGAGCGTGATCACTCAGGCTTGTCCCAAGGTTAGCTTC
GAGCCAATTCCCATTCACTACTGCGCCCCTGCTGGTTTCGCCATCCTGAAGTGTAATAATAAAACATTTA
ACGGAACCGGTCCGTGCACCAACGTGTCAACCGTCCAGTGTACGCATGGCATCCGACCTGTGGTTTCAAC
TCAATTGTTGCTGAACGGCTCCTTGGCCGAGGAGGAGGTAGTCATCAGAAGCGTGAACTTCACCGATAAT
GCCAAGACAATTATAGTGCAGCTGAATACAAGTGTTGAGATCAATTGTACCAGACCAAACAATAACACCC
GCAAACGCATCCGCATCCAGAGAGGTCCTGGCCGGGCATTTGTGACGATCGGAAAGATCGGAAATATGCG
ACAAGCCCACTGCAACATCTCCCGCGCGAAATGGAACAACACTCTCAAACAGATTGCCTCCAAACTCCGA
GAGCAATTCGGGAACAACAAGACAATTATTTTCAAACAGAGTTCTGGCGGCGATCCAGAAATAGTGACTC
ACTCATTTAATTGTGGCGGGGAGTTTTTCTACTGCAACAGCACCCAGCTCTTCAACTCTACCTGGTTTAA
CAGCACTTGGTCCACAGAAGGCAGTAATAATACAGAGGGCTCAGACACAATTACACTGCCTTGCAGAATT
AAACAAATAATAAATATGTGGCAGAAGGTCGGTAAGGCAATGTATGCTCCGCCTATCTCTGGCCAAATTA
GATGTAGTTCAAACATAACCGGGCTTCTCTTGACCCGGGATGGTGGTAATTCTAATAACGAGAGCGAAAT
CTTCAGACCCGGGGGCGGGGACATGCGGGACAACTGGCGGAGCGAGCTCTATAAATATAAGGTGGTGAAG
ATCGAACCCTTGGGAGTCGCCCCAACGAAAGCTAAACGGAGAGTAGTCCAACGCGAGAAGCGGGCTGTAG
GAATAGGCGCCCTGTTTCTTGGGTTCCTCGGGGCCGCCGGCTCAACTATGGGTGCCGCATCCATGACACT
CACCGTGCAGGCACGCCAATTGCTGTCAGGGATCGTTCAGCAGCAGAATAACCTCCTGCGCGCGATAGAG
GCTCAGCAGCATCTGCTGCAGCTGACCGTATGGGGCATCAAGCAGTTGCAGGCCCGCATCTTGGCGGTTG
AGCGGTACCTTAAAGATCAGCAGCTGCTCGGGATCTGGGGATGCAGTGGGAAGTTGATCTGTACAACAGC
AGTGCCCTGGAACGCTTCTTGGTCAAACAAGTCTCTGGAACAAATTTGGAATCATACGACCTGGATGGAG
TGGGACAGAGAGATTAACAACTATACGTCCCTTATTCACTCCCTGATTGAAGAAAGTCAGAATCAGCAGG
AGAAGAACGAACAAGAACTGCTTGAACTTGACAAGTGGGCCTCCCTTTGGAATTGGTTCAATATCACTAA
TTGGCTGTGGTATATAAAGCTGTTCATCATGATTGTGGGGGGCTTGGTCGGGTTGCGGATCGTTTTCGCA
GTGCTGTCAATCGTGAATAGGGTTCGCCAGGGTTACTCCCCTCTGAGCTTTCAGACTCACCTCCCTACCC
CGAGAGGACCTGACAGGCCCGAGGGCATTGAAGAAGAGGGGGGAGAGAGGGACAGAGACAGATCCATCAG
GCTGGTGAACGGGTCACTTGCCCTGATATGGGATGATCTGAGAAGCCTTTGCTTGTTCTCCTATCACAGG
TTGCGGGACTTGCTCCTGATAGTGACCAGGATTGTTGAGTTGCTGGGGCGCCGGGGTTGGGAGGCCCTGA
AGTACTGGTGGAATTTGCTGCAGTACTGGTCACAAGAGCTGAAAAATAGCGCCGTTTCTCTTCTGAATGC
AACTGCCATTGCAGTGGCCGAGGGCACCGATAGGGTTATCGAAGTGGTTCAGGGCGCCTGTAGGGCTATC
CGACATATTCCGCGCCGCATCCGCCAGGGGCTGGAGCGCATCCTGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>VC101721 representing NP_057856
Red=Cloning sites Green=Tags

MRVKEKYQHLWRWGWRWGTMLLGMLMICSATEKLWVTVYYGVPVWKEATTTLFCASDAKAYDTEVHNVWA
THACVPTDPNPQEVVLVNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNT
NSSSGRMIMEKGEIKNCSFNISTSIRGKVQKEYAFFYKLDIIPIDNDTTSYKLTSCNTSVITQACPKVSF
EPIPIHYCAPAGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEEVVIRSVNFTDN
AKTIIVQLNTSVEINCTRPNNNTRKRIRIQRGPGRAFVTIGKIGNMRQAHCNISRAKWNNTLKQIASKLR
EQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNSTQLFNSTWFNSTWSTEGSNNTEGSDTITLPCRI
KQIINMWQKVGKAMYAPPISGQIRCSSNITGLLLTRDGGNSNNESEIFRPGGGDMRDNWRSELYKYKVVK
IEPLGVAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIE
AQQHLLQLTVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWME
WDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFA
VLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSLALIWDDLRSLCLFSYHR
LRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELKNSAVSLLNATAIAVAEGTDRVIEVVQGACRAI
RHIPRRIRQGLERILL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_001802
ORF Size 2568 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_001802.1, NP_057856
RefSeq ORF 2568 bp
Locus ID 155971
UniProt ID P04585
MW 97.2 kDa
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Resources
"NC_001802" in other vectors (19)
SKU Description Size Price
VC101716 Myc-DDK-tagged ORF clone of viral ORF for Tat [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057853 10 ug
$289.00
VC101717 Myc-DDK-tagged ORF clone of viral ORF for Rev [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057854 10 ug
$289.00
VC101718 Myc-DDK-tagged ORF clone of viral ORF for Pr55(Gag) [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057850 10 ug
$289.00 $457.00
VC101719 Myc-DDK-tagged ORF clone of viral ORF for Vif [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057851 10 ug
$289.00 $300.00
VC101720 Myc-DDK-tagged ORF clone of viral ORF for Vpr [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057852 10 ug
$289.00
VC101722 Myc-DDK-tagged ORF clone of viral ORF for Nef [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057857 10 ug
$289.00 $300.00
VC101723 Myc-DDK-tagged ORF clone of viral ORF for Vpu [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057855 10 ug
$289.00
VC101724 Myc-DDK-tagged ORF clone of viral ORF for matrix [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579876 10 ug
$225.00
VC101725 Myc-DDK-tagged ORF clone of viral ORF for capsid [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579880 10 ug
$450.00
VC101726 Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579881 10 ug
$225.00
VC101727 Myc-DDK-tagged ORF clone of viral ORF for p2 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579882 10 ug
$289.00
VC101728 Myc-DDK-tagged ORF clone of viral ORF for p6 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579883 10 ug
$225.00
VC101729 Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579893 10 ug
$289.00
VC101730 Myc-DDK-tagged ORF clone of viral ORF for Envelope transmembrane glycoprotein gp41 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579895 10 ug
$289.00 $457.00
VC101732 Myc-DDK-tagged ORF clone of viral ORF for retropepsin [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_705926 10 ug
$225.00
VC101735 Myc-DDK-tagged ORF clone of viral ORF for p1 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_787042 10 ug
$289.00
VC101736 Myc-DDK-tagged ORF clone of viral ORF for Gag-Pol Transframe peptide [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_787043 10 ug
$289.00
VC101737 Myc-DDK-tagged ORF clone of viral ORF for reverse transcriptase p51 subunit [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_789739 10 ug
$289.00 $457.00
VC101739 Myc-DDK-tagged ORF clone of viral ORF for Envelope surface glycoprotein gp120 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579894 10 ug
$289.00 $457.00

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