rev (NC_001802) Virus Tagged ORF Clone

  • TrueORF®
SKU
VC101717
Myc-DDK-tagged ORF clone of viral ORF for Rev [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057854
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Virus Tagged ORF Clone
Target Symbol rev
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>The Viral ORF clone VC101717 represents NCBI reference of NP_057854 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGGAGAAGTGGCGACAGTGATGAAGAACTGATCCGGACCGTCCGGCTGATTAAGCTCCTGTATC
AGAGCAACCCCCCACCAAATCCAGAGGGGACCAGACAGGCAAGGAGGAATAGGAGACGCAGATGGCGGGA
GCGACAGCGCCAGATCCATAGTATCAGTGAGAGAATCCTCGGCACTTATCTCGGGAGATCAGCAGAGCCC
GTCCCTCTCCAGCTCCCACCTCTTGAACGATTGACCCTGGACTGTAACGAGGATTGTGGTACGAGCGGAA
CCCAGGGCGTCGGCAGTCCACAGATTCTCGTGGAAAGCCCGACTGTGCTCGAATCAGGGACAAAGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>VC101717 representing NP_057854
Red=Cloning sites Green=Tags

MAGRSGDSDEELIRTVRLIKLLYQSNPPPNPEGTRQARRNRRRRWRERQRQIHSISERILGTYLGRSAEP
VPLQLPPLERLTLDCNEDCGTSGTQGVGSPQILVESPTVLESGTKE

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NC_001802
ORF Size 348 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NC_001802.1, NP_057854
RefSeq ORF 348 bp
Locus ID 155908
UniProt ID P04585
MW 13.1 kDa
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Documents
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"NC_001802" in other vectors (19)
SKU Description Size Price
VC101716 Myc-DDK-tagged ORF clone of viral ORF for Tat [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057853 10 ug
$289.00
VC101718 Myc-DDK-tagged ORF clone of viral ORF for Pr55(Gag) [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057850 10 ug
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VC101719 Myc-DDK-tagged ORF clone of viral ORF for Vif [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057851 10 ug
$289.00 $300.00
VC101720 Myc-DDK-tagged ORF clone of viral ORF for Vpr [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057852 10 ug
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VC101721 Myc-DDK-tagged ORF clone of viral ORF for Envelope surface glycoprotein gp160, precursor [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057856 10 ug
$589.00 $784.00
VC101722 Myc-DDK-tagged ORF clone of viral ORF for Nef [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057857 10 ug
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VC101723 Myc-DDK-tagged ORF clone of viral ORF for Vpu [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_057855 10 ug
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VC101724 Myc-DDK-tagged ORF clone of viral ORF for matrix [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579876 10 ug
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VC101725 Myc-DDK-tagged ORF clone of viral ORF for capsid [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579880 10 ug
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VC101726 Myc-DDK-tagged ORF clone of viral ORF for nucleocapsid [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579881 10 ug
$225.00
VC101727 Myc-DDK-tagged ORF clone of viral ORF for p2 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579882 10 ug
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VC101728 Myc-DDK-tagged ORF clone of viral ORF for p6 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579883 10 ug
$225.00
VC101729 Myc-DDK-tagged ORF clone of viral ORF for unnamed protein product [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579893 10 ug
$289.00
VC101730 Myc-DDK-tagged ORF clone of viral ORF for Envelope transmembrane glycoprotein gp41 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579895 10 ug
$289.00 $457.00
VC101732 Myc-DDK-tagged ORF clone of viral ORF for retropepsin [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_705926 10 ug
$225.00
VC101735 Myc-DDK-tagged ORF clone of viral ORF for p1 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_787042 10 ug
$289.00
VC101736 Myc-DDK-tagged ORF clone of viral ORF for Gag-Pol Transframe peptide [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_787043 10 ug
$289.00
VC101737 Myc-DDK-tagged ORF clone of viral ORF for reverse transcriptase p51 subunit [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_789739 10 ug
$289.00 $457.00
VC101739 Myc-DDK-tagged ORF clone of viral ORF for Envelope surface glycoprotein gp120 [Human immunodeficiency virus 1], codon optimized for human cell expression, NP_579894 10 ug
$289.00 $457.00

file-alt Citations

Quercetin Overcomes Cisplatin Resistance by Inducing Ferroptosis via the DDB2-AS1/miR-4728-5p/p53 Axis
Li, Luo, Liu et al
Phytother Res (2026)
Cited by 1
AI Summary: OriGene contributed to this study by providing the REV Virus Tagged ORF Clone (VC101717) and TAT Human Tagged ORF Clone (RC216782), which were used for gene-related experiments investigating the molecular mechanisms by which quercetin overcomes cisplatin resistance in NSCLC.
Snippet: The REV Virus Tagged ORF Clone (VC101717) and TAT Human Tagged ORF Clone (RC216782) were obtained from Origene, a prominent supplier of gene- related
Curcumenol triggered ferroptosis in lung cancer cells via lncRNA H19/miR-19b-3p/FTH1 axis
Zhang, Pan, Xiang et al
Bioact Mater (2022) 13, 23-36 DOI: 10.1016/j.bioactmat.2021.11.013
Cited by 205
AI Summary: In this study, OriGene products including human H19 overexpression plasmid (RC213307) and H19 shRNA plasmid (TG316886) were used to manipulate lncRNA H19 expression in lung cancer cells, enabling the authors to elucidate the role of the lncRNA H19/miR-19b-3p/FTH1 axis in curcumenol-induced ferroptosis.
FigureFigureFigure
Curcumenol triggered ferroptosis in lung cancer cells via lncRNA H19/miR-19b-3p/FTH1 axis
Zhang, Pan, Xiang et al
Bioactive Materials (2021)
Cited by 205
AI Summary: OriGene products, specifically the TAT Human Tagged ORF Clone (RC216782) and REV Virus Tagged ORF Clone (VC101717), were used in this study to investigate the molecular mechanisms by which curcumenol induces ferroptosis in lung cancer cells.
Snippet: Curcumenol, an effective ingredient of Wenyujin, has been reported that exerted its antitumor potential in a few cancer types. However, the effect and molecular mechanism of curcumenol in lung cancer are largely unknown. Here, we found that curcumenol Save;TAT Human Tagged ORF Clone (RC216782) and REV Virus Tagged ORF Clone (VC101717) were purchased from Origene
Showing 1-3 of 3 papers.
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