pLenti-EF1a-Cas9-IRES-Puro CRISPR Vector
pLenti-EF1a-Cas9-IRES-Puro, Cas9 in lenti vector under EF1a promoter with bicistronic IRES-Puro (10 ug)
Cas9 only vectors
Cas9 only vector with puro selection in lenti backbone
3rd generation Lenti vector, ideal for hard-to-transfect cells Cas9 is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment. IRES-Puro is within the Lentiviral packaging cassette and would allow Puromycin selection in both the transfected and the transduced cells. Lenti particles can be produced using high efficiency lenti packaging kits E. Coli selection: chloramphenicol (34 ug/mL) Download vector sequence *Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.