pLenti-EF1a-Cas9-IRES-Puro CRISPR Vector

CAT#: GE100030

pLenti-EF1a-Cas9-IRES-Puro, Cas9 in lenti vector under EF1a promoter with bicistronic IRES-Puro (10 ug)

Product images

Specifications

Product Data

• Function: Cas9 only vectors
• Features:

  Cas9 only vector with puro selection in lenti backbone

  • 3rd generation Lenti vector, ideal for hard-to-transfect cells
  • Cas9 is driven by EF1a, a constitutive promoter as an alternative of CMV promoter. The latter could be silenced in certain cellular environment.
  • IRES-Puro is within the Lentiviral packaging cassette and would allow Puromycin selection in both the transfected and the transduced cells.
  • Lenti particles can be produced using high efficiency lenti packaging kits
  • E. Coli selection: chloramphenicol (34 ug/mL)
  • Download vector sequence