pT7-Guide-IVT CRISPR Vector

CAT#: GE100025

pT7-Guide-IVT vector for target sequence cloning; IVT to produce gRNA, (10ug)

 Cited in 1 publication.

USD 380.00


Availability*
In Stock

Size
    • 10 ug

Product images

Specifications

Product Data
Function CRISPR/Cas9 T7 Vectors
Features

T7 driven gRNA cloning vector

  • gRNA can be produced via in vitro transcription after the target sequence being cloned
  • BsmB I sites are used for target sequence cloning
  • For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven Cas9 mRNA can be produced from a separate vector, SKU GE100014
  • M13 forward sequencing primer can be used to sequence the target sequence after cloning.

Download vector sequence

Citations (1)

Documents

Frequently bought together (2)
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
    • 500 pmol

USD 108.00


Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
    • 500 pmol

USD 108.00

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
10 percent off protein banner ad
68 Mouse Clones
20%off selected tag antibodies