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pT7-Guide-IVT

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SKU
GE100025
pT7-Guide-IVT vector for target sequence cloning; IVT to produce gRNA, (10ug)
  $470.00
In Stock*

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Specifications
Specifications
Product Data
Function CRISPR/Cas9 T7 Vectors
Features

T7 driven gRNA cloning vector

  • gRNA can be produced via in vitro transcription after the target sequence being cloned
  • BsmB I sites are used for target sequence cloning
  • For mRNA mediated genome editing via microinjection or mRNA transfection, both Cas9 mRNA and gRNA transcript are needed. T7 driven Cas9 mRNA can be produced from a separate vector, SKU GE100014
  • M13 forward sequencing primer can be used to sequence the target sequence after cloning.
Download Vector Sequence
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Documents
Resources

file-alt Citations

Generation of Hoxa11-3XFLAG and Hoxd11-3XFLAG alleles to investigate Hox11 genome-wide binding
Echevarría-Andino, Song, van Ginkel et al
Dev Biol (2025) 524, 210-218 DOI: 10.1016/j.ydbio.2025.05.013
Cited by 1
AI Summary: OriGene's T7-Guide IVT (Cat# SKU GE100025) was used in this study to generate guide RNAs for CRISPR/Cas9-mediated insertion of 3XFLAG tags into Hoxa11 and Hoxd11 loci, enabling the investigation of Hox11 genome-wide binding in mice.
Snippet: T7-Guide IVT Blue Heron Biotech, LLC (Commercially available from OriGene) Cat# SKU GE100025
Two CRISPR/Cas9-mediated methods for targeting complex insertions, deletions, or replacements in mouse
Pineault, Novoa, Lozovska et al
MethodsX (2019) 6, 2088-2100 DOI: 10.1016/j.mex.2019.09.003
Cited by 8
AI Summary: In this paper, OriGene’s pUC57-Kan plasmid was used as a backbone for generating long single-stranded DNA (lssDNA) templates essential for CRISPR/Cas9-mediated genome editing in mice.
FigureFigureFigure
PD-linked CHCHD2 mutations impair CHCHD10 and MICOS complex leading to mitochondria dysfunction
Zhou, Ma, Sun et al
Hum. Mol. Genet. (2018)
AI Summary: OriGene’s pT7-Guide-IVT vector and pT7-Cas9 vector were used to generate CRISPR-Cas9 constructs and produce Cas9 mRNA, enabling the creation of isogenic human stem cell lines harboring PD-associated CHCHD2 mutations in this study.
Snippet: Oligos were annealed and ligated with pre-digested pT7-Guide-IVT vector (Origene), followed with in vitro mRNA. Capped and polyadenylated Cas9 mRNA was obtained from pT7-Cas9 vector (Origene) by mMESSAGE Mmachine T7 ULTRA kit (Thermo fisher scientific
MLL4 prepares the enhancer landscape for Foxp3 induction via chromatin looping
Placek, Hu, Cui et al
Nat. Immunol. (2017) 18 (9), 1035-1045 DOI: 10.1038/ni.3812
AI Summary: OriGene’s pT7-Guide-IVT vector was used to clone sgRNA target sequences for CRISPR/Cas9 genome editing in the study, enabling the investigation of MLL4’s role in Treg cell enhancer regulation.
Snippet: hese four sgRNA target sequences were cloned by OriGene Technology into the pT7-Guide-IVT vector. Injectable sgRNAs were in vitro transcribed using the MEGAshortscript T7 Kit (Life Technologies), and the Cas9 mRNA was in vitro transcribed from plasmid MLM3613 (Addgene #42251) using the mMESSAGE mMACHINE T7 Kit (Life Technologies), as previously reported35. These four sgRNAs were co-injected with C...
FigureFigureFigure
MICU1 Serves as a Molecular Gatekeeper to Prevent In Vivo Mitochondrial Calcium Overload
Liu, Liu, Holmström et al
Cell Rep (2016) 16 (6), 1561-73 DOI: 10.1016/j.celrep.2016.07.011
Cited by 232
AI Summary: OriGene’s gRNA cloning services were used to clone sgRNA sequences into vectors, enabling the generation of sgRNAs essential for creating the MICU1-deficient mouse model studied in this paper.
Snippet: These sequences were cloned into a sgRNA vector using OriGene’s gRNA cloning services and were then used as templates to synthesize sgRNAs using the MEGAshortscript T7 kit (Life Technologies)
Showing 1-5 of 5 papers.
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